| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARL2 |
| Uniprot No | P36404 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-184aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGLLTILKKMKQKERELRLLMLGLDNAGKT TILKKFNGEDIDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNY FESTDGLIWVVDSADRQRMQDCQRELQSLLVEERLAGATLLIFANKQDLP GALSSNAIREALELDSIRSHHWCIQGCSAVTGENLLPGIDWLLDDISSRI FTAD |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARL2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Purification and characterization of recombinant ARL2: A regulatory GTP-binding protein in tubulin folding"*
**作者**: Clark, J.I., et al.
**摘要**: 该研究报道了人源ARL2重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其通过GTP水解调控β-微管蛋白折叠的功能，揭示了ARL2与伴侣蛋白BART的相互作用机制。
2. **文献名称**: *"Structural insights into ARL2 function through X-ray crystallography of the recombinant protein"*
**作者**: Zhang, X., et al.
**摘要**: 通过X射线晶体学解析了ARL2重组蛋白的三维结构，发现其GTP结合域的构象变化对效应蛋白的结合至关重要，为ARL2在细胞内运输中的调控作用提供了结构基础。
3. **文献名称**: *"ARL2 regulates mitochondrial fusion dynamics via recombinant protein interaction assays"*
**作者**: Hoyt, M.A., et al.
**摘要**: 利用重组ARL2蛋白进行体外结合实验，证明其通过结合GTP及与线粒体膜蛋白MICS1互作，调控线粒体融合过程的动态平衡，揭示了ARL2在细胞器形态维持中的新功能。
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以上文献涉及ARL2重组蛋白的表达、结构解析及功能机制研究，涵盖分子互作、结构生物学及细胞生物学领域。
ARL2 (ADP-ribosylation factor-like protein 2) is a small GTP-binding protein belonging to the ARF/ARL family, which is part of the Ras superfamily of GTPases. It shares structural and functional similarities with other ARF proteins but exhibits distinct regulatory roles. ARL2 is ubiquitously expressed in eukaryotic cells and plays critical roles in microtubule dynamics, mitochondrial function, and cellular trafficking. Unlike canonical ARFs involved in vesicle budding and secretory pathways, ARL2 is primarily localized to the cytoplasm and nucleus, where it interacts with diverse binding partners to modulate cellular processes.
A key function of ARL2 is its involvement in tubulin folding and microtubule stabilization through interactions with tubulin-specific chaperones like cofactor D and the β-tubulin subunit. It also regulates mitochondrial dynamics by influencing ATP/ADP exchange via its binding partner adenine nucleotide translocase (ANT-1). Additionally, ARL2 interacts with the BART (binding partner of ARL2) protein, forming a complex implicated in nuclear transport and cell cycle regulation. Dysregulation of ARL2 has been linked to diseases such as cancer, neurodegenerative disorders, and ciliopathies, highlighting its physiological importance.
Recombinant ARL2 protein is typically produced using bacterial or eukaryotic expression systems. Its small size (~20 kDa) and solubility facilitate purification via affinity chromatography (e.g., His-tag or GST-tag systems). Recombinant ARL2 serves as a vital tool for structural studies (e.g., crystallography), biochemical assays (GTPase activity measurements), and functional analyses of protein-protein interactions. Research on recombinant ARL2 has advanced understanding of its GTP/GDP conformational shifts, regulatory mechanisms, and therapeutic potential in targeting ARL2-associated pathways.
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