首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠巨噬细胞炎症蛋白1α(MIP-1α/CCL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL3含量。CCL3作为靶点，背景上它是一种趋化因子，在免疫调节、炎症反应等生理病理过程中发挥重要作用。研究机制方面，主要聚焦其与受体结合激活信号通路，调节免疫细胞迁移和活化，探索其在疾病发生发展中的作用及潜在治疗价值。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于炎症机制研究、免疫调节药物体外实验验证、疾病模型中的趋化因子动态分析等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccl3 ELISA Kit; Mip1a ELISA Kit; Scya3 ELISA Kit; C-C motif chemokine 3 ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; MIP-1-alpha ELISA Kit; Small-inducible cytokine A3 ELISA Kit
缩写：CCL3
Uniprot No.：P50229
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 96-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of rat MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 99-108 | ||||
| EDTA plasma (n=4) | 95 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.1522.0512.1021.9694001.4841.3831.4341.3012000.8880.8670.8780.7451000.5220.5110.5170.384500.3360.3470.3420.209250.2530.2640.2590.12612.50.1950.1990.1970.06400.1320.1330.133 | ||||||
货期：3-5 working days
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