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Human Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit

  • 中文名： 人巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA Kit
货号: IPD-E04662h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人巨噬细胞炎性蛋白1α(MIP-1α/CCL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL3含量。CCL3是一种趋化因子，在免疫反应中发挥重要作用。其背景在于它能介导免疫细胞迁移和活化。研究机制显示，CCL3通过与相应受体结合，激活细胞内信号通路，调控免疫细胞的趋化、增殖等活动，为免疫相关疾病的研究提供靶点。试剂盒检测范围为39 pg/ml- 2500 pg/ml，灵敏度为9.75 pg/ml。适用于体外科研场景，如探究炎症相关信号通路机制、评估疾病模型生物标志物动态变化或筛选药物对炎症因子的调控效应，为免疫学、肿瘤学及病理生理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C C motif chemokine 3 ELISA Kit; CCL 3 ELISA Kit; CCL3 ELISA Kit; CCL3_HUMAN ELISA Kit; Chemokine (C C motif) ligand 3 ELISA Kit; Chemokine C C motif ligand 3 ELISA Kit; Chemokine ligand 3 ELISA Kit; G0/G1 switch regulatory protein 19 1 ELISA Kit; G0/G1 switch regulatory protein 19-1 ELISA Kit; G0S19 1 ELISA Kit; G0S19 1 protein ELISA Kit; Heparin binding chemotaxis protein ELISA Kit; L2G25B ELISA Kit; LD78 alpha ELISA Kit; LD78-alpha(4-69) ELISA Kit; LD78alpha ELISA Kit; Macrophage inflammatory protein 1 alpha ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; macrophage inflammatory protein 1a ELISA Kit; MIP 1 alpha ELISA Kit; MIP 1A ELISA Kit; MIP-1-alpha ELISA Kit; MIP-1-alpha(4-69) ELISA Kit; MIP1 alpha ELISA Kit; MIP1A ELISA Kit; PAT 464.1 ELISA Kit; SCYA 3 ELISA Kit; SCYA3 ELISA Kit; SIS alpha ELISA Kit; SIS beta ELISA Kit; SIS-beta ELISA Kit; Small inducible cytokine A3 ELISA Kit; small inducible cytokine A3 (homologous to mouse Mip-1a) ELISA Kit; Small-inducible cytokine A3 ELISA Kit; Tonsillar lymphocyte LD78 alpha protein ELISA Kit
  • 缩写：
    CCL3
  • Uniprot No.：
    P10147
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 pg/ml - 2500 pg/ml
  • 灵敏度：
    9.75 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    86


    Range %

    81-90


    1:2

    Average %

    99


    Range %

    94-102


    1:4

    Average %

    99


    Range %

    97-101


    1:8

    Average %

    94


    Range %

    90-98


  • 回收率：

    The recovery of human MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    93

    89-96


    EDTA plasma (n=4)

    96

    94-99


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2500

    2.483

    2.468

    2.476

    2.372


    1250

    1.999

    1.923

    1.961

    1.857


    625

    1.256

    1.286

    1.271

    1.167


    312.5

    0.639

    0.691

    0.665

    0.561


    156.25

    0.424

    0.439

    0.432

    0.328


    78

    0.292

    0.279

    0.286

    0.182


    39

    0.196

    0.187

    0.192

    0.088


    0

    0.105

    0.103

    0.104




  • 货期：
    3-5 working days

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