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Human mucosae associated epithelia chemokine,MEC ELISA Kit

  • 中文名： 人粘膜相关上皮趋化因子(MEC/CCL28)ELISA Kit
货号: IPD-E07310h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人粘膜相关上皮趋化因子(MEC/CCL28)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL28含量。CCL28是一种趋化因子，在黏膜组织中表达，与免疫细胞的招募和迁移密切相关。其研究机制主要聚焦于它和受体的相互作用，这些作用在黏膜免疫、肿瘤微环境调节等过程中发挥作用，为相关疾病的治疗提供了潜在靶点。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。适用于体外实验中对感染性疾病模型、炎症反应研究或免疫调控机制探索中的MEC/CCL28表达水平分析，例如评估实验条件下趋化因子分泌动态或验证基因干预效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C C motif chemokine ligand 28 ELISA Kit; C-C motif chemokine 28 ELISA Kit; CC chemokine CCL28 ELISA Kit; CCK 1 ELISA Kit; CCK1 ELISA Kit; CCK1 protein ELISA Kit; CCL 28 ELISA Kit; CCL28 ELISA Kit; CCL28_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 28 ELISA Kit; chemokine (C-C motif) ligand 28 splice variant chi ELISA Kit; MEC ELISA Kit; Mucosae associated epithelial chemokine ELISA Kit; Mucosae-associated epithelial chemokine ELISA Kit; Protein CCK1 ELISA Kit; SCYA28 ELISA Kit; Small inducible cytokine A28 [Precursor] ELISA Kit; small inducible cytokine subfamily A (Cys-Cys); member 28 ELISA Kit; Small-inducible cytokine A28 ELISA Kit
  • 缩写：
    CCL28
  • Uniprot No.：
    Q9NRJ3
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/ml - 5000 pg/ml
  • 灵敏度：
    19.5 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MEC/CCL28 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    89


    Range %

    85-98


    1:2

    Average %

    97


    Range %

    90-105


    1:4

    Average %

    98


    Range %

    92-101


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human MEC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    85-105


    EDTA plasma (n=4)

    99

    94-103


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.534

    2.634

    2.584

    2.451


    2500

    1.953

    1.867

    1.910

    1.777


    1250

    1.284

    1.308

    1.296

    1.163


    625

    0.737

    0.699

    0.718

    0.585


    312

    0.457

    0.431

    0.444

    0.311


    156

    0.271

    0.253

    0.262

    0.129


    78

    0.184

    0.184

    0.184

    0.051


    0

    0.137

    0.129

    0.133




  • 货期：
    3-5 working days

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