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Recombinant Human PGK2 protein

  • 中文名: 磷酸甘油酸酯激酶2(PGK2)重组蛋白
  • 别    名: PGK2;PGKB;Phosphoglycerate kinase 2
货号: PA1000-2368
Price: ¥询价
数量:
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产品详情

纯度>85%SDS-PAGE.
种属Human
靶点PGK2
Uniprot NoP07205
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-417aa
氨基酸序列MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
预测分子量47 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PGK2重组蛋白的3篇参考文献概览(注:以下内容为示例性模拟,实际文献需根据具体数据库检索结果调整):

1. **文献名称**: "Expression and Purification of Recombinant Human PGK2 in Escherichia coli for Functional Studies"

**作者**: Zhang L, et al.

**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人PGK2蛋白,优化了诱导条件以提高可溶性表达,并通过酶活实验证实其磷酸甘油酸激酶活性,为后续精子发生相关研究提供工具。

2. **文献名称**: "Structural Characterization of PGK2 Isoform and Its Role in Spermatogenesis"

**作者**: Tanaka K, et al.

**摘要**: 通过X射线晶体学解析了重组PGK2的三维结构,发现其与体细胞同源物PGK1的关键结构差异,并证明PGK2在精子能量代谢中的特异性功能。

3. **文献名称**: "PGK2 Recombinant Protein Enhances Mitochondrial Function in Germ Cell Lines"

**作者**: Chen X, Wang Y

**摘要**: 利用哺乳动物细胞表达系统制备功能性PGK2重组蛋白,实验显示其能显著提高生殖细胞线粒体ATP生成效率,提示其在男性不育症治疗中的潜在应用价值。

*注:实际研究中建议通过PubMed/Google Scholar以“PGK2 recombinant protein”、“phosphoglycerate kinase 2 expression”为关键词检索最新文献,重点关注蛋白表达系统(如原核/真核)、功能验证方法(酶活检测、细胞模型)等方向的研究。*

背景信息

**Background of PGK2 Recombinant Protein**

Phosphoglycerate kinase 2 (PGK2) is an isoform of the phosphoglycerate kinase enzyme family, primarily involved in glycolysis and energy metabolism. Unlike its ubiquitously expressed counterpart, PGK1. PGK2 is encoded by a retrotransposed gene derived from PGK1 and is specifically expressed in germline cells, particularly during spermatogenesis in mammals. This tissue-specific expression is evolutionarily conserved, reflecting its critical role in male reproductive biology. PGK2 compensates for the transcriptional silencing of PGK1 in late-stage spermatogenic cells, ensuring continued glycolytic ATP production essential for sperm motility and maturation.

Structurally, PGK2 retains the conserved catalytic domains of PGK1 but lacks a functional N-terminal nucleotide-binding site due to evolutionary mutations. Recombinant PGK2 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies on its enzymatic activity, structure-function relationships, and interactions. Research highlights its dual role beyond glycolysis, including involvement in chromatin condensation during spermiogenesis and potential regulatory functions in oxidative stress responses.

PGK2 has garnered interest in reproductive health, cancer biology, and metabolic disorders. Aberrant PGK2 expression has been linked to male infertility, while its ectopic activation in certain cancers suggests a metabolic adaptation for tumor proliferation. Recombinant PGK2 serves as a tool for developing diagnostic assays, exploring infertility mechanisms, and designing targeted therapies. Its study bridges gaps in understanding metabolic plasticity in specialized cells and diseases.

In summary, PGK2 recombinant protein provides insights into cell-specific energy regulation, reproductive biology, and pathological pathways, underscoring its biomedical relevance.

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