首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胱天蛋白酶9(Casp-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Casp-9含量。Casp-9即半胱天冬酶9，在细胞凋亡机制中有重要背景。它是凋亡途径关键成员，作为凋亡启动者，被细胞色素C激活后，能招募相关蛋白形成复合物，激活下游Caspases级联反应，引发细胞凋亡，是肿瘤等疾病研究的潜在靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，可广泛应用于基础科研领域，如凋亡信号通路机制研究、药物开发中的靶点验证或疾病模型中Caspase - 9活性变化的动态监测，为体外实验提供稳定可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Casp-9
Uniprot No.：Q9JHK1
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-106 | |||||
回收率：
| The recovery of rat Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 100 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40001.8461.9461.8961.75220001.4421.5421.4921.34810000.9861.0161.0010.8575000.5460.5560.5510.4072500.3200.3300.3250.1811250.2660.2780.2720.12862.50.2040.2070.2060.06200.1430.1440.144 | ||||||
货期：3-5 working days
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