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产品描述：人胱天蛋白酶7(CASP7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物样本中的CASP7含量。CASP7，全称为胱天蛋白酶7，是一种参与细胞凋亡的半胱氨酸蛋白酶。它位于细胞凋亡的执行阶段，由上游胱天蛋白酶（如CASP8、CASP9）激活，并在细胞死亡刺激下被激活以诱导细胞凋亡。CASP7在多种程序性细胞死亡过程中发挥作用，包括细胞凋亡、细胞焦亡和粒酶介导的死亡。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品可为细胞凋亡通路研究、药物作用靶点筛选或疾病模型中CASP7功能探究提供可靠的数据支持，广泛应用于生命科学基础研究、生物标志物筛选及分子机制解析等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：apoptosis-related cysteine peptidase ELISA Kit; Apoptotic protease Mch-3 ELISA Kit; CASP-7 ELISA Kit; CASP7 ELISA Kit; CASP7_HUMAN ELISA Kit; Caspase-7 subunit p11 ELISA Kit; CMH-1 ELISA Kit; ICE-LAP3 ELISA Kit; ICE-like apoptotic protease 3 ELISA Kit
缩写：CASP7
Uniprot No.：P55210
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CASP7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 85-98 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 95-107 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 83-94 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 90-102 | ||||||
回收率：
| The recovery of human CASP7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 85-96 | |||||
| EDTA plasma (n=4) | 99 | 91-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.2032.1562.1802.02320001.3771.4331.4051.24810000.9020.9150.9090.7525000.5040.5100.5070.3502500.3530.3470.3500.1931250.2720.2660.2690.11262.50.2340.2250.2300.07300.1580.1560.157 | |||||||
货期：3-5 working days
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