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产品描述：人胱天蛋白酶8(Casp-8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物样本中的CASP8含量。CASP8是一种由CASP8基因编码的胱天蛋白酶，参与细胞凋亡调控。其研究机制涉及通过剪切RIPK1抑制细胞程序性坏死，并协同RIPK3/MLKL抑制淋巴细胞减少的免疫缺陷性疾病。CASP8的活性决定了细胞死亡途径之间的可塑性，对免疫系统稳态调控至关重要。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中细胞凋亡信号通路分析、药物干预对Casp - 8活性影响的体外评估，或疾病模型（如肿瘤、神经退行性疾病）中凋亡相关标志物的定量研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ALPS2B ELISA Kit; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein ELISA Kit; Apoptosis related cysteine peptidase ELISA Kit; Apoptotic cysteine protease ELISA Kit; Apoptotic protease Mch-5 ELISA Kit; Apoptotic protease Mch5 ELISA Kit; CAP 4 ELISA Kit; CAP4 ELISA Kit; CASP-8 ELISA Kit; CASP8 ELISA Kit; CASP8_HUMAN ELISA Kit; Caspase 8 ELISA Kit; Caspase 8 apoptosis related cysteine peptidase ELISA Kit; Caspase IIX ELISA Kit; Caspase-8 subunit p10 ELISA Kit; caspase8 ELISA Kit; CED 3 ELISA Kit; FADD Homologous ICE/CED3 Like Protease ELISA Kit; FADD Like ICE ELISA Kit; FADD-homologous ICE/CED-3-like protease ELISA Kit; FADD-like ICE ELISA Kit; FLICE ELISA Kit; FLJ17672 ELISA Kit; ICE-like apoptotic protease 5 ELISA Kit; MACH alpha 1/2/3 protein ELISA Kit; MACH ELISA Kit; MACH beta 1/2/3/4 protein ELISA Kit; MACH5 ELISA Kit; MCH 5 ELISA Kit; MCH5 ELISA Kit; MGC78473 ELISA Kit; MORT1 associated ced 3 homolog ELISA Kit; MORT1 associated CED3 homolog ELISA Kit; MORT1-associated CED-3 homolog ELISA Kit; OTTHUMP00000163717 ELISA Kit; OTTHUMP00000163720 ELISA Kit; OTTHUMP00000163724 ELISA Kit; OTTHUMP00000163725 ELISA Kit; OTTHUMP00000165062 ELISA Kit; OTTHUMP00000165063 ELISA Kit; OTTHUMP00000165064 ELISA Kit; OTTHUMP00000206552 ELISA Kit; OTTHUMP00000206582 ELISA Kit
缩写：CASP8
Uniprot No.：Q14790
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CASP-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 86 | |
| Range % | 80-96 | ||
| 1:2 | Average % | 90 | |
| Range % | 84-102 | ||
| 1:4 | Average % | 95 | |
| Range % | 92-100 | ||
| 1:8 | Average % | 93 | |
| Range % | 83-99 |
回收率：
| The recovery of human CASP-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 87-103 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.2262.2382.2322.136 10001.5131.5461.5301.434 5000.9170.9320.9250.829 2500.5880.5790.5840.488 1250.4060.3940.4000.304 62.50.2510.2370.2440.148 31.250.1390.1330.1360.040 00.0950.0970.096 | ||||||
货期：3-5 working days
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