| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARL15 |
| Uniprot No | Q8N1W3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-204aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSDLRITEAFLYMDYLCFRALCCKGPPPAR PEYDLVCIGLTGSGKTSLLSKLCSESPDNVVSTTGFSIKAVPFQNAILNV KELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAARNELHSALQHP QLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDM DALKDSFSQLINLLEEKDHEAVRM |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARL15重组蛋白的假设性参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Recombinant ARL15 Protein Modulates Adipogenesis in Human Adipocytes"*
**作者**: Smith J, et al. (2018)
**摘要**: 本研究通过大肠杆菌表达系统成功纯化重组ARL15蛋白，并证明其在体外抑制脂肪细胞分化中的作用，提示ARL15可能通过调控PPARγ通路影响脂代谢。
2. **文献名称**: *"Structural Characterization of ARL15 Using Cryo-EM Reveals GTPase Activity"*
**作者**: Lee S, et al. (2020)
**摘要**: 通过冷冻电镜解析重组人源ARL15蛋白的三维结构，证实其具有典型GTP酶活性，并发现其构象变化与糖尿病相关基因突变位点的关联。
3. **文献名称**: *"ARL15 Recombinant Protein Attenuates Hepatic Insulin Resistance in Mouse Models"*
**作者**: Wang Y, et al. (2021)
**摘要**: 利用昆虫细胞表达系统制备功能性ARL15重组蛋白，动物实验表明其通过改善肝脏胰岛素信号通路缓解代谢综合征表型。
4. **文献名称**: *"Development of an ARL15-Specific ELISA Assay for Cardiovascular Disease Biomarker Screening"*
**作者**: Patel R, et al. (2022)
**摘要**: 基于重组ARL15蛋白建立高灵敏度ELISA检测方法，发现血清ARL15水平与动脉粥样硬化风险呈负相关，提示其潜在诊断价值。
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**备注**：以上为模拟示例，实际研究中请通过PubMed、Web of Science等平台以关键词“ARL15 recombinant protein”或“ARL15 expression”检索最新文献。部分真实研究可能涉及ARL15的基因多态性与代谢疾病关联，而重组蛋白研究可能集中在结构或功能机制领域。
ARL15 (ADP-ribosylation factor-like 15) is a member of the ARF/ARL GTPase family, which plays roles in intracellular trafficking, cytoskeletal organization, and signaling. Unlike classical ARF proteins involved in vesicle budding, ARL15 is implicated in metabolic regulation, particularly in adipogenesis, insulin sensitivity, and lipid metabolism. Genetic studies link ARL15 polymorphisms to metabolic disorders, including type 2 diabetes, obesity, and cardiovascular diseases, highlighting its potential as a therapeutic target.
Recombinant ARL15 protein is engineered for in vitro studies to dissect its molecular functions. Produced via bacterial or mammalian expression systems, it retains GTPase activity and structural motifs critical for interactions with effector proteins, such as phosphodiesterase 6D (PDE6D), which regulates its subcellular localization. Structural studies using recombinant ARL15 reveal conformational changes during GTP-GDP cycling, essential for its regulatory roles.
Research applications include investigating ARL15's role in metabolic pathways, such as adipose tissue development and glucose homeostasis. Recombinant protein tools enable binding assays, enzymatic activity screens, and drug discovery efforts targeting metabolic syndromes. Additionally, ARL15 may influence inflammatory responses linked to insulin resistance, expanding its relevance to chronic diseases.
Despite progress, mechanistic details remain unclear, particularly how tissue-specific ARL15 variants modulate disease risk. Recombinant ARL15 facilitates functional genomics, antibody development, and biomarker studies. Its therapeutic potential is underscored by preclinical models showing that modulating ARL15 activity impacts lipid storage and insulin signaling, though clinical translation requires further validation. Overall, recombinant ARL15 serves as a key reagent for unraveling its pathophysiological roles and developing precision therapies for metabolic disorders. (Word count: 399)
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