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Human Caspase 1(Casp-1) ELISA Kit

  • 中文名： 人胱天蛋白酶1(Casp-1)ELISA Kit
货号: IPD-E13025h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人胱天蛋白酶1(Casp-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CASP1含量。CASP1即半胱天冬酶-1，它是炎性半胱天冬酶家族成员。背景上，其与炎症反应密切相关。研究机制方面，它能激活白细胞介素等炎性因子，在炎性小体介导的天然免疫应答里发挥关键作用，也参与细胞焦亡过程，是炎症相关疾病研究的重要靶点。试剂盒检测范围为1.56 ng/ml- 100 ng/ml，灵敏度为0.39 ng/ml。该产品可用于体外研究Casp-1在不同病理生理条件下的表达水平变化，例如炎症信号通路调控机制探索、药物或化合物对炎症小体活性的干预效应评估，以及疾病动物模型中相关生物标志物的动态监测，为炎症相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CASP-1 ELISA Kit; CASP1 ELISA Kit; CASP1_HUMAN ELISA Kit; Caspase 1 ELISA Kit; Caspase-1 subunit p10 ELISA Kit; ICE ELISA Kit; IL-1 beta-converting enzyme ELISA Kit; IL-1BC ELISA Kit; IL1 beta converting enzyme ELISA Kit; IL1B convertase ELISA Kit; Interleukin 1 beta convertase ELISA Kit; Interleukin 1B converting enzyme ELISA Kit; Interleukin-1 beta convertase ELISA Kit; Interleukin-1 beta-converting enzyme ELISA Kit; p45 ELISA Kit
  • 缩写：
    CASP1
  • Uniprot No.：
    P29466
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/ml - 100 ng/ml
  • 灵敏度：
    0.39 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    97


    Range %

    92-103


    1:2

    Average %

    104


    Range %

    99-109


    1:4

    Average %

    101


    Range %

    94-108


    1:8

    Average %

    93


    Range %

    89-98


  • 回收率：

    The recovery of human Casp-1spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    102

    97-108


    EDTA plasma (n=4)

    99

    92-105


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    100

    2.644

    2.634

    2.639

    2.448


    50

    2.082

    2.067

    2.074

    1.884


    25

    1.470

    1.428

    1.449

    1.258


    12.5

    0.955

    0.929

    0.942

    0.751


    6.25

    0.547

    0.521

    0.534

    0.343


    3.12

    0.451

    0.453

    0.452

    0.261


    1.56

    0.325

    0.314

    0.319

    0.129


    0

    0.186

    0.195

    0.191



  • 本试剂盒所含材料：

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    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human Casp-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled Casp-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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