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产品描述：人钙蛋白酶5(CAPN5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CAPN5含量。CAPN5（钙蛋白酶5）是一种钙依赖性半胱氨酸蛋白酶，参与细胞信号传导和多种细胞过程。其突变与新生血管性炎性玻璃体视网膜病变有关，研究其功能有助于理解相关疾病的发生机制。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外培养细胞模型、动物组织样本或体液标本的CAPN5蛋白定量分析，为研究其在疾病发生发展中的作用、药物干预效果评估或分子通路机制探索提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADNIV ELISA Kit; Calpain htra 3 ELISA Kit; Calpain htra-3 ELISA Kit; Calpain-5 ELISA Kit; CAN5_HUMAN ELISA Kit; CAPN 5 ELISA Kit; Capn5 ELISA Kit; nCL 3 ELISA Kit; nCL-3 ELISA Kit; New calpain 3 ELISA Kit; VRNI ELISA Kit
缩写：CAPN5
Uniprot No.：O15484
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CAPN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-91 | |||||
| 1:2 | Average % | 105 | ||||
| Range % | 99-108 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of human CAPN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 84-95 | ||||
| EDTA plasma (n=4) | 95 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20001.9721.9171.9451.78710001.3391.3581.3491.1915000.9790.9560.9680.8102500.7280.7120.7200.5621250.4480.4540.4510.29362.50.3810.3690.3750.21731.250.2610.2770.2690.11100.1600.1560.158 | ||||||
货期：3-5 working days
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