首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人可卡因-苯丙胺调节转录蛋白(Cartpt)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Cartpt含量。人可卡因-苯丙胺调节转录蛋白（Cartpt）是一种下丘脑分泌的内源性神经肽，参与调节摄食、药物成瘾及激素分泌等生物过程。其表达受多种转录因子调控，如CREB和RFX5等，通过影响基因转录活性来调节其功能。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外检测不同样本来源的Cartpt表达差异，可应用于成瘾行为机制、神经退行性疾病模型或精神疾病相关的基础研究，为探索Cartpt在神经信号传导及病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CART ELISA Kit; CART prepropeptide ELISA Kit; CART(42-89) ELISA Kit; CART_HUMAN ELISA Kit; Cartpt ELISA Kit; Cocaine and amphetamine regulated transcript ELISA Kit
缩写：Cartpt
Uniprot No.：Q16568
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Cartpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 84-99 | ||||||
| 1:2 | Average % | 88 | |||||
| Range % | 81-97 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 92-100 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of human Cartpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-98 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.5982.6272.6132.5085002.2482.2962.2722.1672501.8111.7951.8031.6981251.1861.2011.1941.08962.50.6430.6390.6410.53631.20.3680.3590.3640.25915.60.1940.2020.1980.09300.1030.1060.105 | |||||||
货期：3-5 working days
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