首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人补体蛋白9(C9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的C9含量。人补体蛋白9(C9)是补体系统中的重要成分，参与膜攻击复合物（MAC）的形成，对病原体和异常细胞具有溶解作用。其研究背景涉及衰老、神经退行性疾病等，机制上与补体激活、炎症反应和细胞凋亡密切相关。试剂盒检测范围为10.97 pg/mL-8000 pg/mL，适用于科研领域中对补体系统功能的基础研究，如炎症反应机制解析、自身免疫性疾病模型中的补体激活评估，或通过体液样本探索C9在感染、肾脏疾病等病理条件下的生物标志物价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C9; Complement component C9
缩写：C9
Uniprot No.：P02748
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine
检测范围：10.97 pg/mL-8000 pg/mL
灵敏度：10.97 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:2000 | Average % | 96 | ||||
| Range % | 90-104 | |||||
| 1:4000 | Average % | 91 | ||||
| Range % | 87-98 | |||||
| 1:8000 | Average % | 95 | ||||
| Range % | 90-110 | |||||
| 1:16000 | Average % | 96 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of human C9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-101 | ||||
| EDTA plasma (n=4) | 97 | 87-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.5772.5142.5462.4552666.71.8451.8641.8551.764888.91.2391.2131.2261.135296.30.8510.8440.8480.75798.80.3880.3990.3940.30332.90.2140.2070.2110.12010.970.1390.1420.1410.05000.0890.0930.091 | ||||||
货期：3-5 working days
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