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产品描述：人补体蛋白C8α(C8A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C8A含量。人补体蛋白C8α（C8A）是补体系统膜攻击复合物（MAC）的核心组分，参与细胞膜攻击，在固有免疫和炎症反应中起关键作用。其研究机制涉及C8A与C9的结合及自聚合，形成靶细胞膜上的孔状结构，以介导细胞裂解。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品可应用于免疫系统相关的基础研究，如探究补体激活通路调控机制、炎症性疾病模型中的补体功能变化，或评估实验条件下（如药物处理、基因编辑）生物样本中C8α的浓度波动。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C8A ELISA Kit; CO8A_HUMAN ELISA Kit; Complement component 8 subunit alpha ELISA Kit; Complement component 8, alpha polypeptide ELISA Kit; Complement component C8 alpha chain ELISA Kit
缩写：C8A
Uniprot No.：P07357
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C8A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| 1:2000 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:4000 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:8000 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of human C8A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 94-102 | |||||
| EDTA plasma (n=4) | 104 | 100-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected1002.5632.4722.5182.373502.2722.1692.2212.076251.7291.6841.7071.56212.51.2531.2931.2731.1286.250.7850.8010.7930.6483.120.4640.4620.4630.3181.560.3160.3200.3180.17300.1410.1480.145 | |||||||
货期：3-5 working days
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