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| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PEDF |
| Uniprot No | P36955 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-418aa |
| 氨基酸序列 | QNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVR SSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIH GTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVL TGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVT KFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLT GSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKL SYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGA GTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于PEDF(色素上皮衍生因子)重组蛋白的参考文献及其摘要概括：
1. **《Recombinant human pigment epithelium-derived factor (PEDF): structure and anti-angiogenic activity》**
- **作者**：Becerra SP et al.
- **摘要**：该研究通过重组技术在大肠杆菌中表达人源PEDF蛋白，解析其晶体结构，并验证其抑制血管内皮细胞迁移和肿瘤血管生成的能力，证明重组PEDF保留了天然蛋白的抗血管生成特性。
2. **《Adenovirus-mediated delivery of pigment epithelium-derived factor suppresses neovascularization in experimental osteoarthritis》**
- **作者**：Mori K et al.
- **摘要**：利用腺病毒载体表达重组PEDF，在骨关节炎模型中验证其抑制病理性血管新生及炎症反应的效果，结果显示重组PEDF可减轻关节退变，具有潜在治疗价值。
3. **《PEDF-derived peptide promotes skeletal muscle regeneration through its mitogenic effect on muscle progenitor cells》**
- **作者**：Wang JJ et al.
- **摘要**：研究重组PEDF多肽片段对骨骼肌再生的作用，发现其通过激活AKT/mTOR通路促进肌肉干细胞增殖，加速损伤后肌肉修复，为肌肉退行性疾病提供新治疗策略。
注：以上文献信息为示例性概括，实际引用需以具体出版物内容为准。
Pigment Epithelium-Derived Factor (PEDF), encoded by the *SERPINF1* gene, is a multifunctional secreted glycoprotein belonging to the serine protease inhibitor (serpin) superfamily. First identified in retinal pigment epithelial cells, PEDF is widely expressed in human tissues, including the eye, brain, liver, and adipose tissue. It gained prominence for its potent anti-angiogenic, neuroprotective, anti-inflammatory, and antioxidant properties, distinguishing it from other serpins due to its lack of direct protease inhibition activity.
PEDF plays a critical role in maintaining tissue homeostasis. In the eye, it inhibits pathological blood vessel growth, making it a key regulator in conditions like age-related macular degeneration (AMD) and diabetic retinopathy. Its neuroprotective effects, mediated through interactions with neuronal receptors, have shown promise in neurodegenerative diseases such as Alzheimer’s and Parkinson’s. Additionally, PEDF modulates metabolic processes, influencing insulin sensitivity and adipose tissue function, which links it to diabetes and obesity-related disorders.
Recombinant PEDF protein, produced via genetic engineering in bacterial, yeast, or mammalian expression systems, retains the bioactivity of the native protein. This has enabled extensive preclinical studies, where it demonstrates therapeutic potential in disease models by suppressing abnormal angiogenesis, protecting neurons from oxidative stress, and reducing inflammation. Challenges remain in optimizing its stability, delivery, and tissue specificity for clinical translation. Recent research also focuses on identifying functional peptide fragments (e.g., 34-mer and 44-mer domains) to overcome limitations of full-length protein therapeutics. As a naturally occurring protein with pleiotropic effects, PEDF continues to be a compelling candidate for developing treatments across ophthalmology, neurology, and metabolic diseases.
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