关键信息
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基因名
IL2Ra
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简介
IL-2R alpha (CD25) is an essential component of high-affinity IL-2 receptors. IL-2R alpha enhances binding of IL-2 to its receptor complex so that regulates T cell growth and other lymphoid functions[1][2]. IL-2R alpha/CD25 Protein, Human (HEK293, Fc) is a recombinant human IL-2R alpha protein with an hFc tag at the C-terminus and is expressed in HEK293 cells. It consists of 192 amino acids (E22-C213).
- 应用
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生物活性
1. 5 µg/mL (100 µL/well) of immoblized recombinant human IL-2 can bind human CD25/IL-2Rα-Fc with a linear range of 1.2-11 ng/mL. 2. Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e. The ED50 this effect is 0.1982 μg/mL in the presence of 30 ng/mL of recombinant human IL-2, corresponding to a specific activity is 5.05×103 units/mg. 3.Immobilized Human IL-2RA-Fc at 5 μg/mL (100 μl/well) can bind Human IL-2-His.The ED50 is 17.52 ng/mL. Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e. The ED50 this effect is 0.1982 μg/mL in the presence of 30 ng/mL of recombinant human IL-2, corresponding to a specific activity is 5.05×103 units/mg.
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别名
rHuIL-2R α/CD25, Fc Chimera; TAC antigen; IL-2-RA; IL-2 R alpha; IL-2Rα; IL2RA
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种属
Human
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表达系统
HEK293
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标签
C-hFc
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纯度
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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蛋白编号
P01589
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表达区间
E22-C213
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氨基酸序列
ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC
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蛋白长度
Partial
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分子量
60-80 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
IL2Ra, also known as the interleukin-2 receptor alpha chain, plays a crucial role in the immune system by modulating the activity of interleukin-2 (IL-2), a key cytokine involved in T-cell proliferation and differentiation. The IL2Ra receptor is primarily expressed on activated T cells, and its function is pivotal in maintaining immune homeostasis and tolerance. Dysregulation or overexpression of IL2Ra has been implicated in various autoimmune diseases, cancers, and immunodeficiencies. Given its central role in immune responses, IL2Ra has emerged as a target for therapeutic interventions. Researchers have focused on the development of recombinant IL2Ra proteins for several purposes, including enhancing our understanding of immune regulation, designing novel immunotherapies, and as potential biomarkers for disease progression. Advances in recombinant DNA technology have facilitated the production of high-purity IL2Ra proteins, allowing for in-depth studies of their structure and function. These efforts aim to elucidate the underlying mechanisms of IL2Ra signaling pathways and discover new strategies to modulate immune responses in pathological conditions. As a result, the research surrounding IL2Ra recombinant proteins is rapidly evolving, providing insights that could lead to innovative approaches in treating immune-related disorders.
