| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDZK1 |
| Uniprot No | Q5T2W1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-519aa |
| 氨基酸序列 | MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQVVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKELGQSQKEQGLSDNILSPVMNGGVQTWTQPRLCYLVKEGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVLADDHLIEVNGENVEDASHEEVVEKVKKSGSRVMFLLVDKETDKRHVEQKIQFKRETASLKLLPHQPRIVEMKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEAGLKNNDLVVAVNGESVETLDHDSVVEMIRKGGDQTSLLVVDKETDNMYRLAHFSPFLYYQSQELPNGSVKEAPAPTPTSLEVSSPPDTTEEVDHKPKLCRLAKGENGYGFHLNAIRGLPGSFIKEVQKGGPADLAGLEDEDVIIEVNGVNVLDEPYEKVVDRIQSSGKNVTLLVCGKKAYDYFQAKKIPIVSSLADPLDTPPDSKEGIVVESNHDSHMAKERAHSTASHSSSNSEDTEM |
| 预测分子量 | 61.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDZK1重组蛋白的3篇参考文献示例(注：文献信息为模拟概括，仅供参考)：
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1. **文献名称**：*"Cloning and functional characterization of PDZK1: A novel scaffold protein regulating lipid metabolism"*
**作者**：Kocher O. et al.
**摘要**：该研究通过重组技术在大肠杆菌中表达了人源PDZK1蛋白，并验证其作为支架蛋白的功能。实验表明，PDZK1通过PDZ结构域与SR-BI(清道夫受体B类I型)相互作用，调控胆固醇吸收和转运，为心血管疾病机制研究提供基础。
2. **文献名称**：*"PDZK1 interacts with MAPKAPK2 and modulates cellular stress signaling pathways"*
**作者**：Gisler S.M. et al.
**摘要**：本研究利用重组PDZK1蛋白进行体外结合实验，发现其与MAPKAPK2激酶的直接相互作用，揭示了PDZK1在细胞应激反应(如氧化压力)中的调控作用，提示其在癌症信号通路中的潜在价值。
3. **文献名称**：*"Structural analysis of PDZK1 domains and their role in protein-protein interaction networks"*
**作者**：Hirakawa T. et al.
**摘要**：通过X射线晶体学解析了重组PDZK1蛋白的PDZ结构域三维结构，阐明其结合特定靶蛋白的分子机制，为设计靶向PDZK1相互作用的药物提供结构基础。
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**备注**：以上文献信息为示例性概括，实际引用需以具体数据库(如PubMed、Web of Science)检索结果为准。建议通过关键词“PDZK1 recombinant protein”或“PDZK1 protein interaction”进一步筛选近期研究。
**Background of PDZK1 Recombinant Protein**
PDZK1 (Post-synaptic density protein 95/Discs large/Zona occludens 1-based protein kinase 1) is a scaffold protein belonging to the PDZ domain-containing protein family. It is characterized by four PDZ domains, which are protein-protein interaction modules that facilitate the assembly of multi-protein complexes by binding to specific C-terminal motifs or internal sequences of target proteins. PDZK1 is predominantly expressed in epithelial tissues, including the kidney, liver, and intestine, where it plays critical roles in regulating membrane transporters, receptors, and signaling pathways.
Functionally, PDZK1 acts as an adaptor protein, organizing and stabilizing membrane-associated proteins. For instance, it interacts with ion transporters (e.g., Na⁺/H⁺ exchanger regulatory factor 1), receptors (e.g., HDL receptor SCARB1), and enzymes to modulate their localization, stability, and activity. In the kidney, PDZK1 is essential for maintaining electrolyte homeostasis by regulating transporters in the proximal tubule. In the liver, it influences lipid metabolism through interactions with HDL-processing proteins. Dysregulation of PDZK1 has been linked to metabolic disorders, hypertension, and cancer, highlighting its physiological and pathological relevance.
Recombinant PDZK1 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to enable in vitro studies. Its purified form allows researchers to investigate protein interactions, structural properties, and mechanistic roles in cellular processes. This tool has advanced understanding of PDZK1's involvement in disease pathways and its potential as a therapeutic target.
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