Recombinant Human ARL1 protein

中文名： ADP核糖基化因子样蛋白1(ARL1)重组蛋白
别名： ARL1；ADP-ribosylation factor-like protein 1

货号: PA1000-218DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	> 90 % SDS-PAGE.
种属	Human
靶点	ARL1
Uniprot No	Q96RJ2
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-196aa
氨基酸序列	MGSSHHHHHH SSGLVPRGSH MGSVNSRGHK AEAQVVMMGL DSAGKTTLLY KLKGHQLVET LPTVGFNVEP LKAPGHVSLT LWDVGGQAPL RASWKDYLEG TDILVYVLDS TDEARLPESA AELTEVLNDP NMAGVPFLVL ANKQEAPDAL PLLKIRNRLS LERFQDHCWE LRGCSALTGE GLPEALQSLW SLLKSRSCMC LQARAHGAER GDSKRS
预测分子量	24 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ARL1重组蛋白的3篇参考文献概览(注：文献信息为示例性整合，实际引用时请核实原文)：

1. **文献名称**: *"Purification and characterization of recombinant ARL1: A role in Golgi membrane dynamics"*

**作者**: Rosenwald, A.G. et al.

**摘要**: 该研究通过大肠杆菌系统重组表达并纯化了ARL1蛋白，发现其GTP结合活性对高尔基体膜结构的维持至关重要，并揭示了ARL1与COPI囊泡运输的潜在关联。

2. **文献名称**: *"ARL1 interacts with GRIP-domain proteins to regulate Golgi localization"*

**作者**: Lu, L., Hong, W.

**摘要**: 作者利用重组ARL1蛋白进行体外结合实验，证实其通过GTP依赖的方式与GBF1等GRIP结构域蛋白互作，调控高尔基体相关蛋白的定位与膜运输功能。

3. **文献名称**: *"Functional analysis of ARL1 in endosomal trafficking using recombinant mutants"*

**作者**: Setty, S.R. et al.

**摘要**: 研究通过构建ARL1重组突变体(如Q71L组成型激活型、T31N显性失活型)，结合细胞成像技术，揭示了ARL1在晚期内体到高尔基体逆向运输中的调控作用。

4. **文献名称**: *"ARL1重组蛋白在酵母中的表达及其脂质结合特性"*

**作者**: Christis, C., Munro, S.

**摘要**: 该文在酵母模型中重组表达ARL1.发现其通过N端两亲性螺旋结构域与特定磷脂结合，调控细胞内膜结构的形态与功能，为ARL1在真核生物中的保守机制提供证据。

(注：以上内容为领域典型研究方向的概括性整合，非真实文献标题，实际文献需通过PubMed/Google Scholar检索确认。)

背景信息

ARL1 (ADP-ribosylation factor-like protein 1) is a member of the ARF/ARL family of small GTPases within the Ras superfamily. These proteins regulate membrane trafficking, organelle structure, and intracellular signaling by cycling between active GTP-bound and inactive GDP-bound states. ARL1. specifically, localizes predominantly to the Golgi apparatus and plays a critical role in maintaining Golgi integrity, vesicle-mediated transport, and lipid droplet dynamics. It interacts with effector proteins like golgins and guanine nucleotide exchange factors (GEFs) to coordinate cargo sorting and membrane tethering processes.

Recombinant ARL1 protein is engineered for in vitro studies to dissect its molecular mechanisms, including GTPase activity, binding partners, and structural dynamics. Produced via heterologous expression systems (e.g., E. coli, insect cells), it retains post-translational modifications when expressed in eukaryotic hosts, enhancing functional relevance. Researchers use recombinant ARL1 to investigate its role in diseases linked to Golgi dysfunction, such as neurodegenerative disorders and cancer, where aberrant membrane trafficking contributes to pathology. Its structure-function relationships, resolved via X-ray crystallography or cryo-EM, provide insights into conserved GTPase regulatory motifs and guide therapeutic targeting. Additionally, ARL1’s involvement in lipid metabolism and autophagy has spurred interest in metabolic disease research. The recombinant form enables high-throughput screening for small-molecule modulators, offering potential pathways for drug development. Overall, ARL1 recombinant protein serves as a vital tool for unraveling cellular logistics and disease mechanisms tied to membrane trafficking networks.