首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人溴结构域睾丸特异蛋白(BRDT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BRDT含量。BRDT是一种溴结构域蛋白。它与染色质的动态变化、基因表达调控密切相关。在研究机制上，BRDT可通过识别乙酰化组蛋白，调控精子发生等生理过程。目前，针对BRDT的研究旨在开发相关靶向药物，用于癌症、生殖疾病等的治疗。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于探索BRDT在睾丸发育、精子形成异常或生殖相关疾病模型中的表达变化，也可用于评估药物或环境因素对生殖细胞表观遗传调控的影响，为生殖医学基础研究、雄性生育力评估及生殖毒性机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BRDT ELISA Kit; Bromodomain testis-specific protein ELISA Kit; Cancer/testis antigen 9 ELISA Kit; CT9 ELISA Kit; RING3-like protein ELISA Kit
缩写：BRDT
Uniprot No.：Q58F21
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BRDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:4 | Average % | 105 | |||||
| Range % | 101-113 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of human BRDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.7512.5932.6722.54120001.8751.7581.8171.68610001.0401.0701.0550.9245000.5920.6110.6020.4712500.3640.3490.3570.2261250.2390.2270.2330.10262.50.1580.1650.1620.03100.1270.1340.131 | |||||||
货期：3-5 working days
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