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产品描述：人丝氨酸/苏氨酸蛋白激酶B-raf(BRAF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物样本中的BRAF含量。BRAF是一种原癌基因，其编码的蛋白在MAPK信号通路中发挥关键作用。该通路参与细胞生长、增殖和存活等过程。BRAF突变可使通路持续激活，导致肿瘤发生。目前针对BRAF靶点已开展大量研究，开发出多种靶向药物用于癌症治疗。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于体外研究BRAF在肿瘤发生机制、信号通路调控或药物干预实验中的表达水平变化，为癌症相关基础研究、分子靶点筛选及生物标志物探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ95109 ELISA Kit; 94 kDa B raf protein ELISA Kit; B raf 1 ELISA Kit; B raf ELISA Kit; B Raf proto oncogene serine threonine protein kinase ELISA Kit; B Raf proto oncogene; serine/threonine kinase ELISA Kit; B RAF1 ELISA Kit; B-Raf proto-oncogene serine/threonine-protein kinase (p94) ELISA Kit; BRAF 1 ELISA Kit; BRAF ELISA Kit; BRAF_HUMAN ELISA Kit; BRAF1 ELISA Kit; cRmil ELISA Kit; MGC126806 ELISA Kit; MGC138284 ELISA Kit; Murine sarcoma viral (v-raf) oncogene homolog B1 ELISA Kit; Murine sarcoma viral v raf oncogene homolog B1 ELISA Kit; NS7 ELISA Kit; Oncogen BRAF ELISA Kit; oncogene BRAF1 ELISA Kit; p94 ELISA Kit; Proto-oncogene B-Raf ELISA Kit; Proto-oncogene c-Rmil ELISA Kit; RAFB 1 ELISA Kit; RAFB1 ELISA Kit; RMIL ELISA Kit; Serine/threonine-protein kinase B-raf ELISA Kit; v raf murine sarcoma viral oncogene homolog B ELISA Kit; v raf murine sarcoma viral oncogene homolog B1 ELISA Kit; v-Raf murine sarcoma viral oncogene homolog B1 ELISA Kit
缩写：BRAF
Uniprot No.：P15056
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BRAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 80-100 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human BRAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-104 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4082.4322.4202.27910001.6591.6761.6681.5275001.0421.0041.0230.8822500.6600.6390.6500.5091250.4290.4240.4270.28662.50.2890.2750.2820.14131.250.2140.2290.2220.08100.1450.1370.141 | ||||||
货期：3-5 working days
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