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产品描述：人溴结构域蛋白4(BRD4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BRD4含量。BRD4，即溴结构域蛋白4，是BET家族成员之一，通过识别乙酰化组蛋白和非组蛋白参与多种生理过程。其异常功能与多种疾病相关，如癌症。BRD4通过结合乙酰化组蛋白调节基因转录，影响细胞周期、细胞分化和信号转导。研究其分子机制有助于开发新型治疗策略。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于科研领域如肿瘤发生机制研究、表观遗传学调控分析、药物开发中的靶点活性评估等场景，尤其适用于细胞模型或动物模型中BRD4表达水平与功能相关性研究，为探索其在疾病中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Brd4 ELISA Kit; BRD4-NUT FUSION ELISA Kit; BRD4-NUT fusion oncoprotein ELISA Kit; BRD4_HUMAN ELISA Kit; Bromodomain containing 4 ELISA Kit; bromodomain containing protein 4 ELISA Kit; Bromodomain-containing protein 4 ELISA Kit; CAP ELISA Kit; chromosome associated protein ELISA Kit; HUNK1 ELISA Kit; HUNKI ELISA Kit; MCAP ELISA Kit; Mitotic chromosome-associated protein ELISA Kit; Protein HUNK1 ELISA Kit
缩写：BRD4
Uniprot No.：O60885
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BRD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 80-93 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 83-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human BRD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-107 | ||||
| EDTA plasma (n=4) | 85 | 80-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12001.9631.9031.9331.7796001.6241.5731.5991.4453001.2171.1821.2001.0461500.9520.9340.9430.789750.5710.5260.5490.39537.50.3350.3170.3260.17218.750.2060.1990.2030.04900.1590.1480.154 | ||||||
货期：3-5 working days
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