| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDGFRa |
| Uniprot No | P16234 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-680aa |
| 氨基酸序列 | MGTSHPAFLVLGCLLTGLSLILCQLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQTIPFNVYALKATSELDLEMEALKTVYKSGETIVVTCAVFNNEVVDLQWTYPGEVKGKGITMLEEIKVPSIKLVYTLTVPEATVKDSGDYECAARQATREVKEMKKVTISVHEKGFIEIKPTFSQLEAVNLHEVKHFVVEVRAYPPPRISWLKNNLTLIENLTEITTDVEKIQEIRYRSKLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQVPSSILDLVDDHHGSTGGQTVRCTAEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHSRDRSTVEGRVTFAKVEETIAVRCLAKNLLGAENRELKLVAPTLRSELTVAAAVLVLLVIVIISLIVLVVIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFPRDGLVLGRVLGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGPHLNIVNLLGACTKSGPIYIITEYCFYG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDGFRα重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Crystal structure of the dimeric ectodomain of PDGFRα in complex with PDGF-AA*
**作者**：Yarden Y et al.
**摘要**：该研究解析了PDGFRα胞外段与配体PDGF-AA结合的晶体结构，揭示了受体二聚化及配体识别的分子机制，为靶向PDGFRα的信号干预提供结构基础。
2. **文献名称**：*Functional characterization of recombinant PDGFRα tyrosine kinase domain: Activation and inhibition studies*
**作者**：Shim AH et al.
**摘要**：通过重组表达PDGFRα的酪氨酸激酶结构域，研究其自磷酸化活性及小分子抑制剂的作用，证实了ATP结合口袋的关键残基对激酶活性的调控。
3. **文献名称**：*PDGFRα mutations in human cancers: Role of recombinant receptor mutants in oncogenic signaling*
**作者**：Heldin CH et al.
**摘要**：利用重组PDGFRα突变体分析肿瘤相关突变(如D842V)对下游信号通路的组成性激活作用，揭示了突变受体在胃肠道间质瘤中的致癌机制。
4. **文献名称**：*Recombinant PDGFRα extracellular domain as a tool for ligand-binding assays and drug discovery*
**作者**：Thompson JE et al.
**摘要**：开发了可溶性重组PDGFRα胞外段蛋白，用于高通量筛选靶向PDGF/PDGFRα相互作用的抑制剂，为抗纤维化及抗肿瘤药物研发提供平台。
(注：以上文献为示例，实际引用需根据具体研究选择并核实原文信息。)
Platelet-Derived Growth Factor Receptor Alpha (PDGFRα) is a transmembrane tyrosine kinase receptor critical for regulating cell proliferation, migration, survival, and differentiation. It belongs to the PDGFR family, which also includes PDGFRβ. PDGFRα binds to PDGF ligands (e.g., PDGF-AA, PDGF-BB, PDGF-CC) with varying affinities, initiating downstream signaling cascades such as RAS-MAPK, PI3K-AKT, and JAK-STAT pathways. These pathways are essential in embryonic development, tissue repair, and angiogenesis. Dysregulation of PDGFRα signaling is linked to cancers, fibrosis, and developmental disorders.
Recombinant PDGFRα proteins are engineered in vitro, typically using mammalian expression systems (e.g., HEK293 or CHO cells), to mimic the extracellular ligand-binding domain or full-length receptor. These proteins retain functional epitopes for ligand interaction and are purified for research or therapeutic applications. They are widely used to study receptor-ligand binding kinetics, screen inhibitors, or investigate signaling mechanisms in cell-based assays. In cancer research, recombinant PDGFRα helps identify oncogenic mutations (e.g., in gastrointestinal stromal tumors) and validate targeted therapies. Additionally, it serves as an immunogen for antibody development or a standard in diagnostic assays. Engineered variants (e.g., soluble receptors or tagged proteins) enhance experimental flexibility for structural studies or high-throughput drug discovery.
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