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| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDGFB |
| Uniprot No | P01127 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 82-190aa |
| 氨基酸序列 | SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT |
| 预测分子量 | 28.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDGFB重组蛋白的3篇参考文献及其摘要概述：
1. **《Platelet-derived growth factor: Structure, function and implications in normal and malignant cell growth》**
- **作者**：Heldin, C.H., & Westermark, B.
- **摘要**：该综述详细解析了PDGF家族(包括PDGFB)的结构与生物学功能，讨论了重组PDGFB在调控细胞增殖、迁移及血管生成中的作用，并探讨其在癌症和纤维化疾病中的病理机制。
2. **《PDGF-BB enhances endothelial cell survival and signaling via interaction with the PDGF β-receptor》**
- **作者**：Ostman, A., et al.
- **摘要**：研究通过重组PDGFB蛋白实验，揭示了其与PDGF β受体的特异性结合机制，阐明了其在促进内皮细胞存活和血管生成中的关键信号通路，为靶向治疗提供理论依据。
3. **《Recombinant human PDGF-BB stimulates fibroblast proliferation and accelerates wound healing in animal models》**
- **作者**：Grotendorst, G.R., et al.
- **摘要**：该文献报道了重组人PDGFB蛋白在动物模型中的应用，证明其能显著促进成纤维细胞增殖，并加速皮肤伤口愈合过程，支持其在临床组织修复中的潜在价值。
(注：以上文献信息为示例性质，实际引用时需核对具体论文的准确性。)
**Background of PDGFB Recombinant Protein**
Platelet-derived growth factor subunit B (PDGFB) is a key member of the PDGF family, a group of signaling proteins that regulate cell growth, differentiation, and tissue repair. Discovered in the 1970s, PDGFs are secreted as disulfide-linked dimers, with PDGFB forming homodimers (PDGF-BB) or heterodimers with PDGFA (PDGF-AB). PDGFB binds to tyrosine kinase receptors (PDGFR-α/β), activating downstream pathways like MAPK and PI3K/AKT, which drive cellular proliferation, migration, and angiogenesis.
PDGFB plays critical roles in embryonic development, wound healing, and vascular homeostasis. It is notably expressed by endothelial cells and platelets, promoting pericyte recruitment during blood vessel formation. Dysregulation of PDGFB signaling is linked to pathologies, including cancer, fibrosis, and atherosclerosis, due to excessive cell proliferation or stromal activation.
Recombinant PDGFB is produced using biotechnological systems (e.g., *E. coli*, mammalian cells*) to ensure high purity and bioactivity. It is widely utilized in research to study mechanisms of angiogenesis, tissue regeneration, and disease progression. Clinically, PDGFB-based therapies, such as becaplermin (Regranex®), a recombinant PDGF-BB gel, have been approved for treating diabetic foot ulcers by stimulating fibroblast and endothelial cell activity.
Despite its therapeutic potential, prolonged PDGFB exposure raises concerns about oncogenic risks, necessitating controlled application. Ongoing studies explore engineered PDGFB variants and targeted delivery systems to enhance efficacy while minimizing off-target effects. As a tool in regenerative medicine and cancer biology, PDGFB recombinant protein remains pivotal in bridging basic research and clinical innovation.
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