| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE6D |
| Uniprot No | O43924 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-150aa |
| 氨基酸序列 | MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural basis of the interaction between the Arf-like GTPase Arl3 and PDEδ"**
*Authors: Hanzal-Bayer M, Renault L, Wittinghofer A*
**摘要**: 该研究解析了重组PDE6D蛋白与Arl3 GTPase的复合物晶体结构，揭示了PDE6D通过疏水口袋介导与异戊二烯化靶蛋白结合的分子机制，为理解细胞内蛋白运输机制提供结构基础。
2. **"PDE6D mediates trafficking of prenylated proteins across the olfactory epithelium"**
*Authors: Zhang X, et al.*
**摘要**: 通过重组PDE6D蛋白功能实验，证明其作为分子伴侣调控嗅觉神经元中异戊二烯化信号蛋白(如Ras家族)的膜定位，缺失PDE6D导致嗅觉功能异常。
3. **"PDE6D binds to the C-terminal tail of RIG-I to facilitate its localization and antiviral signaling"**
*Authors: Huang C, et al.*
**摘要**: 研究发现重组PDE6D蛋白与天然免疫受体RIG-I的C端结构域结合，促进其线粒体定位并增强抗病毒信号通路，扩展了PDE6D在非视觉系统中的功能认知。
4. **"Comparative analysis of PDE6D and UNC119A as cargo transporters for prenylated proteins"**
*Authors: Wätzlich D, et al.*
**摘要**: 利用重组蛋白对比研究，揭示PDE6D与UNC119A在转运异戊二烯化蛋白时的底物偏好性及调控差异，为相关疾病靶点开发提供依据。
(注：以上文献为示例性内容，实际引用需以具体论文为准。)
**Background of PDE6D Recombinant Protein**
Phosphodiesterase 6D (PDE6D) is a regulatory subunit of the cyclic guanosine monophosphate (cGMP)-specific phosphodiesterase family, primarily known for its role in phototransduction within retinal photoreceptor cells. Unlike catalytically active PDE6 isoforms, PDE6D lacks enzymatic activity but functions as a prenyl-binding protein, facilitating the trafficking and membrane localization of prenylated proteins, including components of the visual signaling pathway.
Structurally, PDE6D belongs to the Ras-association domain superfamily and interacts with small GTPases, such as Arl2/Arl3. to regulate the subcellular distribution of lipid-modified proteins. This interaction is critical for maintaining photoreceptor integrity and function. Mutations in PDE6D have been linked to inherited retinal degenerative diseases, including retinitis pigmentosa, underscoring its importance in retinal health.
Recombinant PDE6D protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce high-purity, functional protein for research. Its recombinant form enables detailed biochemical studies, structural analyses (e.g., X-ray crystallography), and exploration of its role in disease mechanisms. Notably, PDE6D has gained attention in cancer research due to its interaction with oncogenic Ras proteins, influencing Ras membrane localization and signaling—a potential therapeutic target in Ras-driven malignancies.
Overall, PDE6D recombinant protein serves as a vital tool for dissecting molecular pathways in vision and cancer, offering insights into therapeutic strategies for related disorders.
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