| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDCL |
| Uniprot No | Q13371 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-301aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMTTLDD KLLGEKLQYY YSSSEDEDSD HEDKDRGRCA PASSSVPAEA ELAGEGISVN TGPKGVINDW RRFKQLETEQ REEQCREMER LIKKLSMTCR SHLDEEEEQQ KQKDLQEKIS GKMTLKEFAI MNEDQDDEEF LQQYRKQRME EMRQQLHKGP QFKQVFEISS GEGFLDMIDK EQKSIVIMVH IYEDGIPGTE AMNGCMICLA AEYPAVKFCK VKSSVIGASS QFTRNALPAL LIYKGGELIG NFVRVTDQLG DDFFAVDLEA FLQEFGLLPE KEVLVLTSVR NSATCHSEDS DLEID |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDCL重组蛋白的示例参考文献(注：以下内容为虚构示例，仅用于演示格式，实际文献需通过学术数据库检索)：
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1. **文献名称**: "Recombinant PDCL3 Protein Enhances Cell Cycle Progression via CDK2 Interaction"
**作者**: Smith A, et al.
**摘要**: 本研究通过在大肠杆菌中表达并纯化重组PDCL3蛋白，探究其与细胞周期蛋白CDK2的相互作用。实验表明PDCL3通过结合CDK2促进G1/S期转换，提示其在细胞周期调控中的关键作用。
2. **文献名称**: "Structural Characterization of PDCL Recombinant Protein and Its Role in Proteasome Assembly"
**作者**: Lee J, et al.
**摘要**: 利用X射线晶体学解析了重组PDCL蛋白的三维结构，发现其C端结构域对蛋白酶体组装至关重要。该研究为PDCL功能机制及疾病相关突变研究提供了结构基础。
3. **文献名称**: "PDCL Recombinant Protein as a Biomarker in Cancer: Expression Analysis and Clinical Implications"
**作者**: Zhang Y, et al.
**摘要**: 通过重组PDCL蛋白制备抗体，检测其在多种肿瘤组织中的表达水平。结果显示PDCL高表达与乳腺癌患者不良预后显著相关，提示其作为潜在生物标志物的价值。
4. **文献名称**: "Functional Study of PDCL in Viral Infection Using Recombinant Protein-Based Assays"
**作者**: Garcia-Sastre M, et al.
**摘要**: 研究利用昆虫细胞系统表达PDCL重组蛋白，发现其通过调控宿主免疫信号通路抑制病毒复制，为抗病毒治疗提供了新靶点。
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**备注**：以上文献信息为模拟示例，实际研究中请通过PubMed、Web of Science等平台检索最新文献。如需具体领域文献，建议结合PDCL的全称及研究方向(如细胞生物学、癌症、免疫学等)优化关键词。
**Background of PDCL Recombinant Protein**
PDCL (Phosducin-like protein) recombinant protein is derived from the PDCL gene family, which encodes regulatory proteins involved in modulating G protein-coupled receptor (GPCR) signaling pathways. These proteins share structural homology with phosducin, a photoreceptor-specific protein known to interact with Gβγ subunits of heterotrimeric G proteins, thereby influencing signal transduction. PDCL proteins are ubiquitously expressed and play critical roles in cellular processes such as protein folding, chaperone activity, and cytoskeletal organization. Their ability to bind Gβγ subunits suggests involvement in diverse physiological functions, including vision, neurotransmission, and immune response regulation.
Recombinant PDCL proteins are typically produced using expression systems like *E. coli* or mammalian cell cultures, enabling large-scale purification for research and therapeutic applications. The recombinant form retains functional domains essential for interacting with client proteins, making it valuable for studying GPCR signaling mechanisms, protein-protein interactions, and chaperone-assisted folding pathways.
PDCL has garnered attention in disease research, particularly in cancer and neurodegenerative disorders, where dysregulated GPCR signaling or protein misfolding is implicated. For instance, PDCL overexpression has been linked to tumor progression, while its deficiency may contribute to neurodegenerative pathologies. Additionally, recombinant PDCL serves as a tool for drug screening, aiding the development of targeted therapies to restore disrupted signaling or proteostasis.
Despite progress, challenges remain in understanding isoform-specific roles (e.g., PDCL1. PDCL2. PDCL3) and post-translational modifications affecting function. Ongoing studies aim to elucidate its structural dynamics and therapeutic potential, positioning PDCL recombinant protein as a pivotal resource in both basic and translational biomedical research.
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