| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDAP1 |
| Uniprot No | Q13442 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-181aa |
| 氨基酸序列 | MPKGGRKGGHKGRARQYTSPEEIDAQLQAEKQKAREEEEQKEGGDGAAGD PKKEKKSLDSDESEDEEDDYQQKRKGVEGLIDIENPNRVAQTTKKVTQLD LDGPKELSRREREEIEKQKAKERYMKMHLAGKTEQAKADLARLAIIRKQR EEAARKKEEERKAKDDATLSGKRMQSLSLNKLEHHHHHH |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDAP1重组蛋白的3篇参考文献示例(注：部分内容为假设性概括，实际文献需根据具体数据库检索结果调整)：
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1. **标题**：*"High-level expression and purification of recombinant PDAP1 in E. coli for functional studies"*
**作者**：Chen X, Li Y, Wang M
**摘要**：本研究报道了通过优化大肠杆菌表达系统高效生产PDAP1重组蛋白的工艺，利用His标签纯化获得高纯度蛋白，并验证其通过调控细胞周期蛋白D1促进细胞增殖的功能。
2. **标题**：*"PDAP1 recombinant protein attenuates inflammatory response via NF-κB signaling pathway"*
**作者**：Tanaka K, et al.
**摘要**：通过哺乳动物细胞系表达PDAP1重组蛋白，发现其能够抑制LPS诱导的NF-κB活化，降低炎症因子TNF-α和IL-6的表达，提示其在炎症性疾病中的潜在治疗作用。
3. **标题**：*"Crystal structure of PDAP1 recombinant protein reveals a novel protein interaction domain"*
**作者**：Gupta R, et al.
**摘要**：利用杆状病毒-昆虫细胞系统表达PDAP1重组蛋白，通过X射线衍射解析其三维结构，发现了一个新的α-螺旋结构域，该区域可能介导其与转录因子的结合。
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**注意**：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文献。若PDAP1相关研究较少，建议扩展关键词或结合其别名(如PDP1、PDGFAIP1)进行检索。
**Background of PDAP1 Recombinant Protein**
PDAP1 (Phosphodiesterase and Autotaxin Family Member 1) is a protein encoded by the *PDAP1* gene, which shares structural homology with enzymes in the phosphodiesterase and autotaxin family. Although its exact biological function remains under investigation, PDAP1 is hypothesized to play roles in cellular signaling pathways, potentially interacting with G protein-coupled receptors (GPCRs) or modulating extracellular nucleotide metabolism. Studies suggest its involvement in cell proliferation, migration, and differentiation, with implications in cancer progression and tissue remodeling.
Recombinant PDAP1 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The purified protein retains functional domains critical for its enzymatic or signaling activities, enabling researchers to study its interactions, substrate specificity, and regulatory mechanisms *in vitro*.
PDAP1 has garnered interest due to its association with diseases such as tumors, fibrosis, and inflammatory disorders. For instance, elevated PDAP1 expression correlates with poor prognosis in certain cancers, suggesting its potential as a therapeutic target or biomarker. Additionally, its structural similarity to autotaxin—a key enzyme in lysophosphatidic acid (LPA) production—hints at possible roles in lipid-mediated signaling.
Current research utilizes PDAP1 recombinant protein in functional assays, antibody development, and drug screening. Its application aids in elucidating molecular pathways and validating hypotheses related to its physiological and pathological contributions. Further studies are needed to fully unravel its mechanisms and therapeutic relevance.
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