首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨形成蛋白4 (BMP4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的BMP4含量。BMP4即骨形态发生蛋白4，属于TGF-β超家族。它在胚胎发育、骨骼形成等生理过程中扮演重要角色。研究机制方面，BMP4通过与特异性受体结合，激活下游信号通路，调控细胞的增殖、分化和凋亡等生物学行为。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品可广泛应用于体外细胞模型中BMP4分泌水平的动态监测、疾病动物模型血清标志物分析，以及基于体液样本的分子机制研究，为探索BMP4在发育生物学、骨代谢调控及病理生理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：zgc:100779 ELISA Kit; BMP 2B ELISA Kit; BMP 4 ELISA Kit; BMP-2B ELISA Kit; BMP-4 ELISA Kit; BMP2B ELISA Kit; BMP2B1 ELISA Kit; BMP4 ELISA Kit; BMP4_HUMAN ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; Bone morphogenetic protein 4 ELISA Kit; DVR4 ELISA Kit; MCOPS6 ELISA Kit; MGC100779 ELISA Kit; OFC11 ELISA Kit; zbmp-4 ELISA Kit; ZYME ELISA Kit
缩写：BMP4
Uniprot No.：P12644
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 95-107 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 82-94 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human BMP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 86 | 80-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4472.5422.4952.3715001.5241.6281.5761.4522500.9480.8980.9230.7991250.5010.5120.5070.38362.50.3070.3140.3110.18731.20.2560.2450.2510.12715.60.1970.1910.1940.07000.1230.1240.124 | ||||||
货期：3-5 working days
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