首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨形态发生蛋白3(BMP3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BMP3含量。人骨形态发生蛋白3（BMP3）是一种转化生长因子-β超家族成员，具有诱导骨和软骨发育的能力。它通过负向调节骨密度，在成骨祖细胞分化中作为其他BMP的拮抗剂，并在骨折组织中高度表达。BMP3的研究揭示了其在骨再生和发育中的重要作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验模型、动物研究或疾病机制探索中BMP3蛋白动态变化的检测，如骨发育相关基因调控研究、再生医学中成骨分化评估或代谢性疾病生物标志物分析等领域，为深入解析BMP3在生理病理过程中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP-3 ELISA Kit; BMP-3A ELISA Kit; bmp3 ELISA Kit; BMP3_HUMAN ELISA Kit; BMP3A ELISA Kit; Bone morphogenetic protein 3 (osteogenic) ELISA Kit; Bone morphogenetic protein 3 ELISA Kit; Bone morphogenetic protein 3A ELISA Kit; Osteogenin ELISA Kit
缩写：BMP3
Uniprot No.：P12645
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-106 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 86-97 | ||||||
回收率：
| The recovery of human BMP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 99-108 | |||||
| EDTA plasma (n=4) | 92 | 87-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.5122.6332.5732.3995001.8341.9481.8911.7172501.1761.2181.1971.0231250.7560.7740.7650.59162.50.4630.4730.4680.29431.20.2890.2960.2930.11915.60.2250.2350.2300.05600.1730.1750.174 | |||||||
货期：3-5 working days
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