首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠骨成型蛋白2(BMP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BMP2含量。BMP2即骨形态发生蛋白2，是转化生长因子超家族成员。它在骨骼发育、修复等过程中起重要作用。其研究机制主要围绕诱导间充质干细胞向成骨细胞分化展开，能促进骨组织形成，在骨损伤修复、骨疾病治疗等领域有潜在应用价值。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于体外检测实验动物模型中BMP-2的动态变化，例如骨折愈合、骨代谢调控或干细胞定向分化等基础研究领域；为骨生物学、组织工程等研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bmp2 ELISA kit; Bmp-2Bone morphogenetic protein 2 ELISA kit; BMP-2 ELISA kit; Bone morphogenetic protein 2A ELISA kit; BMP-2A ELISA kit
缩写：BMP2
Uniprot No.：P49001
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 95-110 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-104 | |||||
回收率：
| The recovery of rat BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-93 | ||||
| EDTA plasma (n=4) | 93 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.7142.5962.6552.5342502.2122.1422.1772.0561251.4551.5241.4901.36962.50.8000.7890.7950.67431.20.4480.4210.4350.31415.60.2530.2430.2480.1277.80.1720.1850.1790.05800.1230.1180.121 | ||||||
货期：3-5 working days
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