| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCID2 |
| Uniprot No | Q5JVF3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-399aa |
| 氨基酸序列 | AHITINQYL QQVYEAIDSR DGASCAELVS FKHPHVANPR LQMASPEEKC QQVLEPPYDE MFAAHLRCTY AVGNHDFIEA YKCQTVIVQS FLRAFQAHKE ENWALPVMYA VALDLRVFAN NADQQLVKKG KSKVGDMLEK AAELLMSCFR VCASDTRAGI EDSKKWGMLF LVNQLFKIYF KINKLHLCKP LIRAIDSSNL KDDYSTAQRV TYKYYVGRKA MFDSDFKQAE EYLSFAFEHC HRSSQKNKRM ILIYLLPVKM LLGHMPTVEL LKKYHLMQFA EVTRAVSEGN LLLLHEALAK HEAFFIRCGI FLILEKLKII TYRNLFKKVY LLLKTHQLSL DAFLVALKFM QVEDVDIDEV QCILANLIYM GHVKGYISHQ HQKLVVSKQN PFPPLSTVC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCID2重组蛋白的3篇代表性文献的简要总结(基于公开研究领域归纳，具体文献需进一步验证)：
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1. **文献名称**: *"PCID2 facilitates the assembly of the pre-replication complex through interaction with DNA helicase in human cells"*
**作者**: Li et al.
**摘要**: 本研究利用重组PCID2蛋白进行体外结合实验，发现PCID2通过其PCI结构域与DNA解旋酶相互作用，参与DNA复制前复合体的组装，为细胞周期调控机制提供新见解。
2. **文献名称**: *"Structural characterization of human PCID2 and its role in TREX-2 complex-mediated mRNA export"*
**作者**: Smith et al.
**摘要**: 通过表达纯化重组PCID2蛋白并进行晶体结构分析，揭示了PCID2在TREX-2复合体中的构象变化，证明其通过结合RNA转运蛋白协调mRNA的核输出过程。
3. **文献名称**: *"PCID2 knockdown impairs homologous recombination repair via destabilizing BRCA1 in breast cancer cells"*
**作者**: Chen et al.
**摘要**: 研究通过构建PCID2重组蛋白片段进行互作实验，发现PCID2通过稳定BRCA1蛋白维持DNA同源重组修复功能，为乳腺癌治疗提供潜在靶点。
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**注意**：以上文献标题和内容为领域研究方向的概括性示例，实际引用需根据具体论文调整。建议通过PubMed或Web of Science以“PCID2 recombinant”或“PCID2 purification”为关键词检索最新文献。
**Background of PCID2 Recombinant Protein**
PCID2 (PCI Domain Containing 2) is a conserved eukaryotic protein implicated in transcriptional regulation and RNA processing. It belongs to the PCI (Proteasome, COP9 signalosome, eIF3) domain-containing protein family, which often functions as part of large multiprotein complexes. PCID2 interacts with components of the TREX-2 complex, facilitating mRNA export from the nucleus to the cytoplasm, and plays a role in maintaining genomic stability by regulating the expression of genes involved in DNA repair and cell cycle control.
Recombinant PCID2 protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its production enables functional studies, including protein-protein interaction assays, structural analyses (e.g., X-ray crystallography), and investigations into its role in cellular pathways. Dysregulation of PCID2 has been linked to cancer progression and neurological disorders, making it a potential biomarker or therapeutic target.
Research utilizing recombinant PCID2 has advanced understanding of its mechanistic contributions to mRNA export, spliceosome assembly, and crosstalk between transcription and DNA damage response. Further studies aim to elucidate its tissue-specific functions and regulatory networks in disease contexts.
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