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产品描述：人B细胞淋巴瘤因子2(Bcl-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BCL2含量。BCL2是重要的细胞凋亡调节靶点。其背景在于它是抗凋亡蛋白家族成员，异常高表达与多种肿瘤发生发展密切相关。研究机制主要围绕抑制细胞凋亡、维持细胞存活展开，靶向抑制BCL2有望促使癌细胞凋亡，为癌症治疗提供新思路。试剂盒检测范围为0.9 ng/mL-60 ng/mL，适用于癌症发生机制研究、药物开发中的疗效评估或细胞凋亡相关基础实验；为科研人员研究Bcl - 2在肿瘤微环境、耐药性形成或基因调控网络中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BCL2; Apoptosis regulator Bcl-2
缩写：BCL2
Uniprot No.：P10415
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Bcl-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-98 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-108 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of human Bcl-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 91-108 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected602.3662.4652.4162.271301.6591.7581.7091.564151.0081.0271.0180.8737.50.6050.6140.6100.4653.750.3920.4010.3970.2521.80.2580.2680.2630.1180.90.2270.2230.2250.08000.1440.1450.145 | ||||||
货期：3-5 working days
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