首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Bcl-2相关X蛋白(BAX)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BAX含量。BAX是一种促凋亡蛋白，属于Bcl-2家族，通过渗透线粒体外膜启动细胞凋亡。其研究机制涉及BH3依赖和非依赖途径的膜相互作用，BAX激活后可导致线粒体膜通透性改变，释放细胞色素C等凋亡相关分子。试剂盒检测范围为1.25 ng/mL-80 ng/mL，适用于基础研究中BAX蛋白的定量分析，例如细胞凋亡机制探索、药物作用效果评估或疾病模型中的分子表达变化研究。实验操作兼容常规酶标仪检测系统，可满足科研场景下对低丰度BAX蛋白的高灵敏度检测需求，为凋亡相关信号通路研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apoptosis regulator BAX ELISA Kit; BAX ELISA Kit; Bax-protein ELISA Kit; BAX_HUMAN ELISA Kit; BAXA ELISA Kit; Baxdelta2G9 ELISA Kit; Baxdelta2G9omega ELISA Kit; Baxdelta2omega ELISA Kit; Bcl-2-like protein 4 ELISA Kit; BCL2 associated X protein ELISA Kit; BCL2 associated X protein omega ELISA Kit; BCL2 associated X protein transcript variant delta2 ELISA Kit; Bcl2-L-4 ELISA Kit; BCL2L4 ELISA Kit; membrane isoform alpha ELISA Kit
缩写：BAX
Uniprot No.：Q07812
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.312 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 95 | |||||||||||
| Range % | 87-101 | ||||||||||||
| 1:2 | Average % | 94 | |||||||||||
| Range % | 91-103 | ||||||||||||
| 1:4 | Average % | 95 | |||||||||||
| Range % | 92-100 | ||||||||||||
| 1:8 | Average % | 94 | |||||||||||
| Range % | 88-97 | ||||||||||||
回收率：
| The recovery of human BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 102 | 96-109 | |||||||||||
| EDTA plasma (n=4) | 96 | 90-100 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| ng/mlOD1OD2AverageCorrected 802.3052.2152.2602.108 401.6751.7651.7201.568 201.1641.1721.1681.016 100.7950.8090.8020.650 50.4530.4470.4500.298 2.50.3220.3360.3290.177 1.250.2250.2190.2220.070 00.1520.1510.152 | |||||||||||||
货期：3-5 working days
×
