首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人BMP激活素膜结合抑制因子同源物(BAMBI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BAMBI含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外培养细胞模型、动物组织样本或生物液体中BAMBI表达水平的动态监测，为研究其在器官纤维化、肿瘤发生发展或干细胞分化等领域的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BAMBI ELISA Kit; BAMBI_HUMAN ELISA Kit; BMP and activin membrane bound inhibitor homolog ELISA Kit; BMP and activin membrane-bound inhibitor homolog ELISA Kit; NMA ELISA Kit; Non metastatic gene A protein ELISA Kit; Non-metastatic gene A protein ELISA Kit; Putative transmembrane protein NMA ELISA Kit
缩写：BAMBI
Uniprot No.：Q13145
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BAMBI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
| 1:2 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-99 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of human BAMBI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-93 | |||||
| EDTA plasma (n=4) | 97 | 92-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.3242.4952.4102.28651.5511.6301.5911.4672.50.9500.9040.9270.8031.250.5720.5420.5570.4330.6250.3630.3790.3710.2470.3120.2640.2830.2740.1500.1560.2240.2160.2200.09600.1270.1210.124 | |||||||
货期：3-5 working days
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