首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠骨碱性磷酸酶(BALP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆、血浆样本中的BALP含量。BALP即骨碱性磷酸酶，它是成骨细胞的表型标志物。其背景在于它参与骨代谢过程，能反映成骨细胞活性及骨形成情况。研究机制为通过检测其在血液等中的含量变化，可辅助诊断与骨代谢相关疾病，如佝偻病、骨质疏松等。试剂盒检测范围为15.6 U/L-1000 U/L，骨代谢机制研究、骨质疏松模型评估、骨折愈合进程监测、骨骼相关药物干预实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpl ELISA Kit; Alkaline phosphatase ELISA Kit; tissue-nonspecific isozyme ELISA Kit; AP-TNAP ELISA Kit; TNSALP ELISA Kit; EC 3.1.3.1 ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit
缩写：BALP
Uniprot No.：P08289
种属：Rattus norvegicus (Rat)
样本类型：serum, tissue homogenates, plasma
检测范围：15.6 U/L-1000 U/L
灵敏度：3.9 U/L
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-96 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 96-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-104 | |||||
回收率：
| The recovery of rat BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-99 | ||||
| EDTA plasma (n=4) | 95 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| U/LOD1OD2AverageCorrected10002.5612.3752.4682.3015001.8541.8861.8701.7032501.2721.2341.2531.0861250.8050.8170.8110.64462.50.5240.5540.5390.37231.20.3620.3530.3580.19115.60.2630.2580.2610.09400.1700.1630.167 | ||||||
货期：3-5 working days
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