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产品描述：人β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、脑脊液（CSF）样本中的Aβ1-42含量。Aβ1-42 是备受关注的靶点。它是β淀粉样蛋白的一种形式，在阿尔茨海默病患者脑中大量沉积形成斑块。研究机制上，聚焦其产生、聚集过程及与神经毒性、神经炎症等关联，旨在通过干预Aβ1-42 来探索治疗神经退行性疾病的方法。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域中对阿尔茨海默病模型构建、药物干预效果评估、脑脊液生物标志物动态监测等研究，尤其适合探究中枢神经系统或外周体液中Aβ1 - 42的病理生理变化，为神经退行性疾病的分子机制及治疗策略开发提供实验支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
缩写：Aβ1-42
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.3022.3792.3412.244 101.7621.7811.7721.675 51.1241.1891.1571.060 2.50.6530.6790.6660.569 1.250.3750.3980.3870.290 0.6250.2510.2590.2550.158 0.3120.1510.1530.1520.055 00.0950.0980.097 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human Aβ1-42 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled Aβ1-42 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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