首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠β淀粉样蛋白1-40(Aβ1-40)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Aβ1-40含量。Aβ1-40是重要的阿尔茨海默病相关靶点。Aβ蛋白异常聚集形成的斑块是该病典型病理特征，Aβ1-40是Aβ的一种常见亚型。研究机制主要集中在揭示其产生、聚集过程及对神经细胞毒性作用，以探索治疗AD的新策略。试剂盒检测范围为0.625 pg/mL-40 pg/mL，该产品可广泛应用于神经科学领域的基础研究，例如评估疾病模型中Aβ1 - 40的病理积累水平、探究药物干预对淀粉样蛋白代谢的影响，或通过体液检测分析个体化模型的生物标志物动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
缩写：Aβ1-40
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 pg/mL-40 pg/mL
灵敏度：0.156 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 105 | ||||
| Range % | 100-113 | |||||
回收率：
| The recovery of rat Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected402.7062.5762.6412.484202.1812.0742.1281.971101.5121.5021.5071.35051.1071.0501.0790.9222.50.6240.6690.6470.4901.250.4730.4470.4600.3030.6250.3150.2990.3070.15000.1590.1550.157 | ||||||
货期：3-5 working days
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