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产品描述：人β淀粉样蛋白1-40(Aβ1-40)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆、脑脊液（CSF）样本中的Aβ1-40含量。Aβ1-40是β-淀粉样蛋白的一种，在阿尔茨海默病研究中备受关注。其在大脑中异常聚集形成淀粉样斑块，是该病的病理特征之一。研究机制主要聚焦于探究其生成、清除过程的失衡，以及它对神经元毒性作用等方面，以寻找治疗AD的新靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL，可广泛应用于神经退行性疾病机制研究、药物开发中淀粉样蛋白调控效果评估、以及脑脊液生物标志物谱分析等科研领域，为探索Aβ1 - 40在病理过程中的分子作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
缩写：Aβ1-40
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates, cerebrospinal fluid (CSF)
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 96-110 | |||||
回收率：
| The recovery of human Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.8462.6872.7672.61040002.2372.0892.1632.00620001.6791.6121.6461.48910001.2461.2111.2291.0725000.7360.6970.7170.5602500.5120.4980.5050.3481250.3030.2960.3000.14300.1600.1540.157 | ||||||
货期：3-5 working days
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