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产品描述：人天青杀素/肝素结合蛋白(AZU/HBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆、组织培养上清液样本中的AZU1含量。AZU1，也称为肝素结合蛋白（HBP）和阳离子抗菌蛋白CAP37，是一种由中性粒细胞颗粒释放的抗菌和趋化性糖蛋白，属于肽酶S1家族和弹性酶亚家族。它主要对革兰氏阴性细菌具有细胞毒性，可能参与炎症过程中单核细胞的募集。研究显示，AZU1在急性早幼粒细胞白血病（APL）中表达升高，可能与白血病细胞的分化和治疗反应有关。试剂盒检测范围为7.8 pg/mL-500 pg/mL，支持研究者定量分析AZU/HBP在炎症反应、免疫调控或感染模型中的动态变化，为探索其作为炎症相关生物标志物的功能机制提供可靠工具，广泛应用于免疫学、细胞生物学及疾病机制等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AZU1
Uniprot No.：P20160
种属：Homo sapiens (Human)
样本类型：serum, urine, tissue homogenates, cell culture supernates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AZU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-107 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of human AZU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 80-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 5002.8982.6822.7902.686 2502.5312.4862.5092.405 1252.1472.0452.0961.992 62.51.6021.5011.5521.448 31.20.8870.8720.8800.776 15.60.5040.4930.4990.395 7.80.2780.2560.2670.163 00.1070.1000.104 | ||||||
货期：3-5 working days
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