首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠β-抑制蛋白2(ARRB2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ARRB2含量。ARRB2即β-抑制蛋白2，是细胞内重要信号调节分子。它在G蛋白偶联受体信号通路中发挥关键作用，可介导受体脱敏、内吞等过程。研究发现其与肿瘤、心血管疾病等病理过程相关，通过调控相关信号通路影响疾病发生发展，是潜在治疗靶点。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，适用于基础科研领域，如探究GPCR信号转导机制、细胞功能调控网络，或研究心血管疾病、肿瘤模型中ARRB2的动态表达变化，为受体下游信号研究及药物作用靶点筛选提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Arrb2Beta-arrestin-2 ELISA Kit; Arrestin beta-2 ELISA Kit
缩写：ARRB2
Uniprot No.：P29067
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ARRB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 90-109 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 91-105 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 88-100 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 94-108 | ||||||
回收率：
| The recovery of rat ARRB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 92-106 | |||||
| EDTA plasma (n=4) | 100 | 94-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 30002.4322.3702.4012.220 15002.0011.8961.9491.768 7501.4311.3891.4101.229 3750.8420.8250.8340.653 187.50.4940.4780.4860.305 93.750.3600.3440.3520.171 46.880.2690.2730.2710.090 00.1830.1790.181 | |||||||
货期：3-5 working days
×
