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产品描述：人肌动蛋白相关蛋白2/3复合物亚基2(ARPC2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ARPC2含量。ARPC2是一个重要靶点。它在细胞的多种活动如迁移、黏附等过程中发挥关键作用。相关研究主要围绕其参与的细胞骨架构建机制，探索其在疾病尤其是肿瘤发生、发展中的作用，以寻找新的治疗策略和药物作用位点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于基础科研中探究细胞骨架动态调控机制、肿瘤侵袭转移研究、神经发育及免疫细胞趋化作用等领域的样本分析，为细胞生物学、分子病理学等研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Actin related protein 2/3 complex subunit 2 34kDa ELISA Kit; Actin related protein 2/3 complex subunit 2 ELISA Kit; Actin-related protein 2/3 complex subunit 2 ELISA Kit; ARC 34 ELISA Kit; ARC34 ELISA Kit; Arp2/3 complex 34 kDa subunit ELISA Kit; ARP2/3 protein compex subunit 34 ELISA Kit; ARPC 2 ELISA Kit; Arpc2 ELISA Kit; ARPC2_HUMAN ELISA Kit; p34 Arc ELISA Kit; p34-ARC ELISA Kit; p34Arc ELISA Kit; PNAS 139 ELISA Kit; PNAS139 ELISA Kit; PRO 2446 ELISA Kit; PRO2446 ELISA Kit
缩写：ARPC2
Uniprot No.：O15144
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ARPC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of human ARPC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 90 | 85-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.2012.1222.1622.0117501.5641.4761.5201.3693751.0070.9530.9800.829187.50.6830.6340.6590.508940.4920.4530.4730.322470.3540.3310.3430.19223.50.2460.2390.2430.09200.1550.1460.151 | ||||||
货期：3-5 working days
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