首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人ADP核糖基化因子样蛋白8B(ARL8B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ARL8B含量。ARL8B，全称ADP-核糖基化因子样8B，是一种小GTPase，与溶酶体相关，参与调节细胞内囊泡运输、细胞器定位和溶酶体动力学。研究显示，ARL8B在阿尔茨海默症等神经退行性疾病中可能发挥重要作用，其与β-淀粉蛋白的关联性为诊断提供了新线索。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，科研人员可通过探究ARL8B在神经退行性疾病、肿瘤微环境调控或代谢异常模型中的功能机制，也可用于体外细胞实验中溶酶体相关通路的分子机制研究。为自噬调控、细胞器运输等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADP ribosylation factor like 10C ELISA Kit; ADP ribosylation factor like 8B ELISA Kit; ADP-ribosylation factor-like protein 10C ELISA Kit; ADP-ribosylation factor-like protein 8B ELISA Kit; ARL10C ELISA Kit; ARL8B ELISA Kit; ARL8B_HUMAN ELISA Kit; FLJ10702 ELISA Kit; Gie1 ELISA Kit; Novel small G protein indispensable for equal chromosome segregation 1 ELISA Kit
缩写：ARL8B
Uniprot No.：Q9NVJ2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ARL8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:10 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:20 | Average % | 104 | ||||
| Range % | 99-109 | |||||
| 1:40 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human ARL8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 95-104 | ||||
| EDTA plasma (n=4) | 90 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.6802.7522.7162.538 5001.9792.0762.0281.850 2501.2301.2141.2221.044 1250.7730.7390.7560.578 62.50.4550.4760.4660.288 31.20.3840.3690.3770.199 15.60.2700.2860.2780.100 00.1750.1810.178 | ||||||
货期：3-5 working days
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