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Human Apolipoprotein A2,apo-A2 ELISA Kit

  • 中文名: 人载脂蛋白A2(apo-A2)ELISA Kit
货号: IPD-E13504h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人载脂蛋白A2(apo-A2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的APOA2含量。APOA2是一种载脂蛋白,位于高密度脂蛋白颗粒中,具有胆固醇转移活性,参与动物器官再生、对雌激素和糖皮质激素的反应。其位于细胞外空间,是乳糜微粒、球形高密度脂蛋白颗粒和极低密度脂蛋白颗粒的一部分,与家族性高胆固醇血症有关。试剂盒检测范围为1 ng /mL-800 ng/mL,适用于心血管疾病机制研究、代谢性疾病动物模型分析、药物干预实验或体外组织样本中脂蛋白代谢相关指标的科研检测,检测结果可为探索 apo - A2 在脂质代谢调控、炎症反应及疾病发生发展中的作用提供定量依据本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    APO A2 ELISA Kit; Apo AII ELISA Kit; Apo-AII ELISA Kit; APOA 2 ELISA Kit; ApoA II ELISA Kit; ApoA-II ELISA Kit; APOA2 ELISA Kit; APOA2_HUMAN ELISA Kit; APOAII ELISA Kit; Apolipoprotein A II ELISA Kit; Apolipoprotein A-II(1-76) ELISA Kit; Apolipoprotein A2 ELISA Kit; Apolipoprotein AII ELISA Kit; ApolipoproteinA II ELISA Kit; OTTHUMP00000032244 ELISA Kit
  • 缩写:
    APOA2
  • Uniprot No.:
    P02652
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1 ng /mL-800 ng/mL
  • 灵敏度:
    1 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human apo-A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1000

    Average %

    103


    Range %

    96-110


    1:2000

    Average %

    96


    Range %

    89-103


    1:4000

    Average %

    99


    Range %

    92-105


    1:8000

    Average %

    95


    Range %

    88-101


  • 回收率:

    The recovery of human apo-A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    104

    97-110


    EDTA plasma (n=4)

    98

    93-103


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    800

    2.570

    2.634

    2.602

    2.409


    267

    2.148

    2.067

    2.107

    1.915


    89

    1.627

    1.618

    1.623

    1.430


    30

    1.074

    1.029

    1.051

    0.859


    10

    0.522

    0.531

    0.527

    0.334


    3

    0.444

    0.453

    0.448

    0.256


    1

    0.254

    0.244

    0.249

    0.056


    0

    0.195

    0.190

    0.193




  • 货期:
    3-5 working days

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