首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Apelin受体(APLNR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的APLNR含量。APLNR（Apelin受体）是一种G蛋白偶联受体，其内源性配体为Apelin。该受体参与调节心血管、中枢神经系统、葡萄糖代谢等功能，并在胚胎和肿瘤血管生成中发挥作用。研究显示，APLNR在心肌肥厚和心衰治疗中具有潜在应用价值，其研究机制涉及G蛋白偶联受体信号通路和β-arrestin信号通路。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于体外培养细胞模型中受体信号机制研究、疾病动物模型的组织分析，以及临床前药物开发中靶点作用效果的动态监测；支持科研人员在心血管代谢性疾病机制探索、肿瘤微环境研究或再生医学相关课题中高效获取定量数据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APLNR; AGTRL1; APJ; Apelin receptor; Angiotensin receptor-like 1; G-protein coupled receptor APJ; G-protein coupled receptor HG11
缩写：APLNR
Uniprot No.：P35414
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APLNR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 94-103 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 96-105 | |||||
回收率：
| The recovery of human APLNR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 96 | 94-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.3122.1892.2512.0817501.4941.4551.4751.3053751.0260.9941.0100.840187.50.6550.6880.6720.502940.4480.4420.4450.275470.3030.2920.2980.12823.50.2410.2320.2370.06700.1630.1770.170 | ||||||
货期：3-5 working days
×
