| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARFIP1 |
| Uniprot No | Q9JHU5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-341aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAQESPKNSAAEIPVTSNGEVDDSREH SFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGGQRTQTKSG PVILADEIKNPAMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELE AQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLE LHEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQ YESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRN DVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKL KTPGVDAPSWLEEQ |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"ARFIP1 interacts with ARF1 and regulates Golgi complex dynamics"**
- **作者**: Someya, A., et al.
- **摘要**: 研究揭示了ARFIP1通过其ARF结合结构域与ARF1相互作用，调控高尔基体膜运输的分子机制，重组蛋白实验证实其参与囊泡形成和细胞器结构维持。
2. **"Structural analysis of ARFIP1 reveals a BAR domain protein regulating cellular trafficking"**
- **作者**: Lee, M.C., et al.
- **摘要**: 通过重组ARFIP1蛋白的晶体结构解析，发现其BAR结构域介导膜弯曲功能，并阐明其与ARF GTP酶协同调控内吞和分泌途径的作用。
3. **"Phosphorylation-dependent regulation of ARFIP1 in cancer cell migration"**
- **作者**: Zhang, Y., et al.
- **摘要**: 利用重组ARFIP1蛋白验证其磷酸化修饰(如Ser-132位点)对肿瘤细胞迁移的影响，表明其通过调节细胞骨架重塑参与侵袭转移过程。
4. **"ARFIP1 modulates amyloid precursor protein processing via endosomal pathways"**
- **作者**: Chen, L., et al.
- **摘要**: 通过体外重组ARFIP1与APP的相互作用实验，证明其通过调控内体运输影响β-淀粉样蛋白生成，为阿尔茨海默病机制提供新视角。
(注：以上文献信息为模拟示例，实际引用需以具体论文为准。)
ARFIP1 (ADP-ribosylation factor-interacting protein 1) is a multidomain protein encoded by the *ARFIP1* gene, primarily involved in regulating intracellular vesicular trafficking and cytoskeletal dynamics. It belongs to the ArfGAP protein family, characterized by the presence of an ArfGAP (ADP-ribosylation factor GTPase-activating protein) domain, which modulates the activity of Arf GTPases—key regulators of membrane trafficking and organelle structure. ARFIP1 additionally contains a RhoGAP domain, enabling interaction with Rho GTPases to influence actin cytoskeleton remodeling, cell motility, and signal transduction. Other functional domains include an N-terminal ANK (ankyrin) repeat for protein-protein interactions and a PH (pleckstrin homology) domain that binds phosphoinositides, linking it to membrane-associated processes.
Recombinant ARFIP1 protein is engineered through heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for mechanistic studies. Its recombinant form allows researchers to dissect its dual GAP activities, structural roles in vesicle formation, and crosstalk between Arf and Rho signaling pathways. Studies implicate ARFIP1 in diseases such as cancer, where dysregulated trafficking and cytoskeletal abnormalities contribute to metastasis, and neurological disorders tied to synaptic vesicle transport defects. Recombinant ARFIP1 is also utilized in high-throughput screens to identify small-molecule modulators of Arf/Rho pathways, offering therapeutic potential. Overall, this protein serves as a critical tool for unraveling cellular logistics and developing targeted interventions for trafficking-related pathologies.
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