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产品描述：人DNA-(无嘌呤或无嘧啶位点)裂合酶(APEX1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的APEX1含量。APEX1是重要的DNA修复酶，在碱基切除修复途径中起核心作用。它还具有氧化还原功能，可调节多种转录因子的活性。异常表达与肿瘤的发生、发展及耐药相关，是肿瘤治疗的潜在靶点，目前相关研究聚焦于开发靶向药物抑制其活性。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本产品适用于基础科研中探索DNA损伤修复机制、氧化应激相关疾病模型（如肿瘤、神经退行性疾病）的分子机制研究，或用于体外实验中评估药物处理、基因编辑等干预手段对APEX1表达水平的影响，为相关领域研究提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AP endonuclease 1 ELISA Kit; AP endonuclease class I ELISA Kit; AP lyase ELISA Kit; APE 1 ELISA Kit; APE ELISA Kit; APE-1 ELISA Kit; APEN ELISA Kit; APEX 1 ELISA Kit; APEX ELISA Kit; APEX nuclease (multifunctional DNA repair enzyme) 1 ELISA Kit; Apex nuclease 1 ELISA Kit; APEX nuclease ELISA Kit; APEX1 ELISA Kit; APEX1_HUMAN ELISA Kit; Apurinic endonuclease ELISA Kit; Apurinic-apyrimidinic endonuclease 1 ELISA Kit; Apurinic/apyrimidinic (abasic) endonuclease ELISA Kit; Apurinic/apyrimidinic endonuclease 1 ELISA Kit; Apurinic/apyrimidinic exonuclease ELISA Kit; APX ELISA Kit; BAP1 ELISA Kit; Deoxyribonuclease (apurinic or apyrimidinic) ELISA Kit; DNA (apurinic or apyrimidinic site) lyase ELISA Kit; DNA-(apurinic or apyrimidinic site) lyase; mitochondrial ELISA Kit; EC 4.2.99.18 ELISA Kit; HAP 1 ELISA Kit; HAP1 ELISA Kit; Human Apurinic endonuclease 1 ELISA Kit; MGC139790 ELISA Kit; Multifunctional DNA repair enzyme ELISA Kit; Redox factor 1 ELISA Kit; Redox factor-1 ELISA Kit; REF 1 ELISA Kit; REF 1 protein ELISA Kit; REF-1 ELISA Kit; REF1 ELISA Kit; REF1 protein ELISA Kit
缩写：APEX1
Uniprot No.：P27695
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APEX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 93-108 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 95-107 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 84-95 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 84-95 | ||||||
回收率：
| The recovery of human APEX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 89-105 | |||||
| EDTA plasma (n=4) | 104 | 93-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.2532.1552.2042.0585001.3981.4341.4161.2702500.9160.9000.9080.7621250.5070.4870.4970.35162.50.3510.3660.3590.21331.250.2680.2720.2700.12415.60.2290.2340.2320.08600.1470.1450.146 | |||||||
货期：3-5 working days
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