首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血清淀粉样蛋白P(SAP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APCS含量。APCS即血清淀粉样蛋白C，是一种急性期蛋白。其在炎症反应等生理病理过程中发挥重要作用。研究机制方面，它涉及免疫调节，与炎症信号通路相互作用，还参与组织损伤修复等过程，在疾病诊断和治疗靶点研究中有潜在价值。试剂盒检测范围为7.8 ng/mL-500 ng/mL，支持研究者定量分析不同实验条件下（如疾病模型构建、药物干预等）生物样本的 SAP 表达动态，为探索炎症相关疾病机制、评估生物标志物水平或筛选潜在治疗靶点提供可靠工具，广泛应用于基础医学、病理学及药理学等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：9.5S alpha 1 glycoprotein ELISA Kit; 9.5S alpha-1-glycoprotein ELISA Kit; Amyloid P component serum ELISA Kit; APCS ELISA Kit; MGC88159 ELISA Kit; Pentaxin related ELISA Kit; Pentraxin 2 ELISA Kit; PTX 2 ELISA Kit; PTX2 ELISA Kit; SAMP_HUMAN ELISA Kit; SAP ELISA Kit; Serum Amyloid P ELISA Kit; Serum amyloid P component ELISA Kit; Serum amyloid P-component(1-203) ELISA Kit
缩写：APCS
Uniprot No.：P02743
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 94-103 | |||||
回收率：
| The recovery of human SAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.5392.5222.5312.3792501.8941.9541.9241.7721251.3011.2881.2951.14362.50.7150.7020.7090.55731.20.4440.4620.4530.30115.60.3370.3210.3290.1777.80.2450.2370.2410.08900.1530.1510.152 | ||||||
货期：3-5 working days
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