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产品描述：人酰基氨基酸释放酶(APEH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的APEH含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可通过本产品研究 APEH 在细胞代谢调控、疾病发生机制中的作用，或用于药物开发中靶点活性评估。为体外蛋白水平研究提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AARE ELISA Kit; ACPH ELISA Kit; ACPH_HUMAN ELISA Kit; Acyl peptide hydrolase ELISA Kit; Acyl-peptide hydrolase ELISA Kit; Acylamino acid releasing enzyme ELISA Kit; Acylamino-acid-releasing enzyme ELISA Kit; Acylaminoacyl peptidase ELISA Kit; Acylaminoacyl-peptidase ELISA Kit; APEH ELISA Kit; APH ELISA Kit; N acylaminoacyl peptide hydrolase ELISA Kit; OPH ELISA Kit; Oxidized protein hydrolase ELISA Kit
缩写：APEH
Uniprot No.：P13798
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APEH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 82-87 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:8 | Average % | 108 | |||||
| Range % | 104-112 | ||||||
回收率：
| The recovery of human APEH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 95-103 | |||||
| EDTA plasma (n=4) | 86 | 86-89 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.0492.1022.0761.96010001.6041.5861.5951.4795001.0801.1021.0910.9752500.6510.6720.6620.5461250.4240.4020.4130.29762.50.2620.2710.2670.15131.250.2060.1990.2030.08700.1170.1150.116 | |||||||
货期：3-5 working days
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