首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人活化蛋白C(APC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APC含量。APC（腺瘤性结肠息肉病基因）是一种肿瘤抑制蛋白，作为Wnt信号传导途径的拮抗剂，其突变与家族性腺瘤性息肉病（FAP）和结直肠癌的发生密切相关。研究APC机制主要关注其如何通过调节Wnt通路、细胞迁移、粘附、转录激活和细胞凋亡等过程，影响肿瘤的发生和发展。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于凝血调控机制、炎症相关疾病模型或药物干预等基础研究；在血栓性疾病、败血症及肿瘤微环境等研究领域具有重要价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：APC
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 87-96 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-97 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 94-103 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 87 | 84-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1002.7552.9902.8732.754 502.0652.2012.1332.014 251.3591.2881.3241.205 12.50.8530.8160.8350.716 6.250.4700.4730.4720.353 3.120.3250.3020.3140.195 1.560.1840.1920.1880.069 00.1150.1220.119 | ||||||
货期：3-5 working days
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