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Human apelin 13,AP13 ELISA Kit

  • 中文名： 人apelin 13(AP13)ELISA Kit
货号: IPD-E13072h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人apelin 13(AP13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的AP13含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于体外实验中探索AP13在疾病模型中的表达调控、药物干预效果评估或信号通路研究；为心血管与代谢性疾病的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    AP13
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human AP13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    104


    Range %

    100-108


    1:2

    Average %

    98


    Range %

    91-105


    1:4

    Average %

    101


    Range %

    94-108


    1:8

    Average %

    94


    Range %

    89-100


  • 回收率：

    The recovery of human AP13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    93

    88-99


    EDTA plasma (n=4)

    100

    96-105


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    400

    2.354

    2.434

    2.394

    2.316


    200

    1.326

    1.337

    1.332

    1.253


    100

    0.798

    0.818

    0.808

    0.730


    50

    0.502

    0.529

    0.516

    0.437


    25

    0.249

    0.251

    0.250

    0.172


    12.5

    0.147

    0.153

    0.150

    0.072


    6.25

    0.123

    0.118

    0.121

    0.042


    0

    0.076

    0.081

    0.078




  • 货期：
    3-5 working days

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