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产品描述：人膜联蛋白Ⅴ(ANX-Ⅴ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的ANXA5含量。ANXA5 即膜联蛋白 A5，是一种具有钙依赖性磷脂结合特性的蛋白。其背景上，在细胞凋亡、凝血调节等生理病理过程有重要作用。研究机制方面，它可与凋亡细胞膜上暴露的磷脂酰丝氨酸高亲和力结合，还能在凝血级联反应里抑制磷脂依赖性反应。试剂盒检测范围为0.312 ng/mL-20ng/mL，适用于体外细胞模型中凋亡进程评估、疾病相关分子机制研究或药物干预效应分析等科研场景，为探索ANX - Ⅴ在病理生理过程中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Anchorin CII ELISA Kit; Annexin 5 ELISA Kit; Annexin A5 ELISA Kit; Annexin V ELISA Kit; Annexin-5 ELISA Kit; Annexin5 ELISA Kit; AnnexinA5 ELISA Kit; AnnexinV ELISA Kit; ANX 5 ELISA Kit; ANX A5 ELISA Kit; ANX5 ELISA Kit; ANXA5 ELISA Kit; ANXA5_HUMAN ELISA Kit; Calphobindin I ELISA Kit; CBP-I ELISA Kit; Endonexin II ELISA Kit; ENX 2 ELISA Kit; ENX2 ELISA Kit; Lipocortin V ELISA Kit; PAP I ELISA Kit; PAP-I ELISA Kit; Placental anticoagulant protein 4 ELISA Kit; Placental anticoagulant protein I ELISA Kit; PLACENTAL PROTEIN 4 ELISA Kit; PP 4 ELISA Kit; Pp4 ELISA Kit; RPRGL3 ELISA Kit; Thromboplastin inhibitor ELISA Kit; VAC-alpha ELISA Kit; Vascular anticoagulant-alpha ELISA Kit
缩写：ANXA5
Uniprot No.：P08758
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-V in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:1 | Average % | 107 | ||
| Range % | 96-112 | |||
| 1:2 | Average % | 101 | ||
| Range % | 91-107 | |||
| 1:4 | Average % | 97 | ||
| Range % | 86-103 | |||
| 1:8 | Average % | 103 | ||
| Range % | 95-107 |
回收率：
| The recovery of human ANX-V spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 90-106 | |||||
| EDTA plasma (n=4) | 102 | 95-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 202.7222.6352.6792.556 102.0811.9632.0221.899 51.4551.4011.4281.305 2.50.9480.9170.9330.810 1.250.6230.6080.6160.493 0.6250.3390.3260.3330.210 0.3120.2310.2290.2300.107 00.1250.1210.123 | |||||||
货期：3-5 working days
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